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MABT121 Anti-Vimentin Antibody, clone 3CB2

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MABT121
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch, R, LzWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT121
      DescriptionAnti-Vimentin Antibody, clone 3CB2
      Alternate Names
      • Vimentin
      Background InformationVimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.
      References
      Product Information
      FormatPurified
      Control
      • ARPE cell lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

      Western Blot Analysis:
      A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

      Immunohistochemistry Analysis:
      A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).
      Biological Information
      ImmunogenVimentin extracted from chicken embryonic brain tissue.
      Clone3CB2
      HostMouse
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Chicken
      • Rat
      • Lizard
      Species Reactivity NoteDemonstrated to react with Human.
      Demonstrated to react with Chameleon and Rat based on the following reference:
      GLIA 15:389-400 (1995), Prada, F. A., et. al.
      Predicted to react to Chicken based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VIM
      UniProt Number
      UniProt SummaryFUNCTION: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

      SUBUNIT STRUCTURE: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM.
      Molecular Weight~54 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in ARPE19 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT121 04053252293795

      Documentation

      Anti-Vimentin Antibody, clone 3CB2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vimentin Antibody, clone 3CB2 Certificates of Analysis

      TitleLot Number
      Anti-Vimentin, Clone 3CB2 - 4142003 4142003
      Anti-Vimentin, clone 3CB2 2839589
      Anti-Vimentin, clone 3CB2 - 2540028 2540028
      Anti-Vimentin, clone 3CB2 - 2562386 2562386
      Anti-Vimentin, clone 3CB2 - 3168750 3168750
      Anti-Vimentin, clone 3CB2 - 3225899 3225899
      Anti-Vimentin, clone 3CB2 - 3574548 3574548
      Anti-Vimentin, clone 3CB2 - 3931718 3931718
      Anti-Vimentin, clone 3CB2 - NRG1906477 NRG1906477
      Anti-Vimentin, clone 3CB2 -2592714 2592714

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies