Millipore Sigma Vibrant Logo

AB1032 Anti-Versican Antibody, a.a. 535-598 of mouse versican

View Products on Sigmaaldrich.com
AB1032
50 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIP, WB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1032
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Versican Antibody, a.a. 535-598 of mouse versican
      Alternate Names
      • GAG alpha Domain
      Background InformationVersican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.
      Product Information
      FormatPurified
      Control
      • Immunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain
      PresentationImmunoglobulin was purified by affinity chromatography and is presented in PBS with no preservative Anti-GST has not been adsorbed.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Versican Antibody, a.a. 535-598 of mouse versican is validated for use in IP, WB, IH(P) for the detection of Versican.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (formalin fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.

      Western blotting: 0.5 μg/mL

      Immunoprecipitation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGST fusion protein containing amino acids 535-598 of mouse versican.
      Epitopea.a. 535-598 of mouse versican
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityMouse versican GAG alpha domain
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VCAN
      • GHAP
      • DKFZp686K06110
      • WGN
      • PG-M
      • WGN1
      • ERVR
      • CSPG2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13611 # May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
      SIZE: 3396 amino acids; 372820 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Cerebral white matter. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas; isoform V2 is restricted to normal brain and gliomas; isoform V3 is found in all these tissues except medulloblastomas.
      DEVELOPMENTAL STAGE: Disappears after the cartilage development.
      DISEASE: SwissProt: P13611 # Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
      SIMILARITY: SwissProt: P13611 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB1032 04053252321450