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MABT886 Anti-VE-Cadherin Antibody, clone Vli37

This Anti-VE-Cadherin Antibody, clone Vli37 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of VE-Cadherin.

Anti-VE-Cadherin Antibody, clone Vli37 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: Cdh5 UniProt Number: P55284
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MABT886
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, BWB, IH(P), ICCRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABT886
      DescriptionAnti-VE-Cadherin Antibody, clone Vli37
      Alternate Names
      • Cadherin-5
      • CD144
      • Vascular endothelial cadherin
      • VE-Cad
      • VE-cadherin
      • VEcad
      Background InformationCadherin-5 (UniProt P55284; also known as Vascular endothelial cadherin, VE-Cad, VE-cadherin, VEcad, CD144) is encoded by the Cdh5 gene (Gene ID 12562) in murine species. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin, which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).
      Product Information
      FormatCulture Supernatant
      PresentationRabbit monoclonal IgG in supernatant with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-VE-Cadherin Antibody, clone Vli37 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of VE-Cadherin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse brain endothelial bEnd.3 cell lysate.
      Western Blotting Analysis: An 1:2,000 dilution from a representative lot detected VE-cadherin expression in lysastes from mouse lung tissue, mouse brain endothelial bEnd.3 cells, and bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
      Immunohistochemistry Analysis: An 1:250-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in formalin-fixed, paraffin-embeded mouse lung, aorta, and liver tissue sections (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
      Immunocytochemistry Analysis: An 1:500-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
      Biological Information
      ImmunogenLinear peptide corresponding to the C-terminal cytoplasmic domain sequence of mouse VE-Cadherin.
      EpitopeNear C-terminus.
      CloneVli37
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityClone Vli37 stains endothelial adherens junctions by immunocytochemistry and immunohistochemistry.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Bovine
      Species Reactivity NoteMouse, Bovine. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cdh5
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~110 observed. Target band size appears larger than the calculated molecular weight of 83.05 kDa due to glycosylation.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse lung tissue lysate.

      Western Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABT886 04055977331141