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MABC1765-100UL Anti-TfR1 (CD71) Antibody, clone 3F3-FMA

Anti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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MABC1765-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1765-100UL
      DescriptionAnti-TfR1 (CD71) Antibody, clone 3F3-FMA
      Alternate Names
      • TR
      • TfR
      • TfR1
      • Trfr
      • T9
      • p90
      • CD71
      Background InformationTransferrin receptor protein 1 (UniProt: Q62351; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the Tfrc (also known as Trfr) gene (Gene ID: 7037) in human. TfR1 is a single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-67), a transmembrane domain (aa 68-88), and a long extracellular domain (aa 89-760). It can also be proteolytically cleaved at Arg100 to generate the soluble serum form (sTfR). TfR1 is involved in the uptake of iron via receptor mediated endocytosis into specialized endosomes where acidification leads to release of iron. TfR1 is reported to be essential for erythrocyte development and positive regulation of T and B cell proliferation through iron uptake. It also acts as a lipid sensor that regulates mitochondrial fusion by regulating the activation of the JNK pathway and degradation of mitofusin MFN2. Under conditions of low dietary levels of stearate (C18:0), it promotes the activation of JNK pathway that results in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2 and inhibition of mitochondrial fusion. Clone 3F3-FMA is reported to be an effective ferroptosis-staining agent. It stains plasma membrane and the perinuclear region associated with the Golgi and the endosomal recycling compartment. (Ref.: Senilymaz, D., et al. (2015). Nature. 525(7567); 124-128; Feng, H., et al. (2020). Cell Rep. 30(10); 3411-3423).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesFlow Cytometry Analysis: A representative lot detected TfR1 (CD71) in Flow Cytometry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TfR1 (CD71) in HT-1080 cells.

      Immunohistochemistry Applications: A representative lot detected TfR1 (CD71) in Immunohistochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Immunocytochemistry Analysis: A representative lot detected TfR1 (CD71) in Immunocytochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Western Blotting Analysis: A representative lot detected TfR1 (CD71) in Western Blotting applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified membrane fractions from OCI-LY7 cells.
      EpitopeUnknown
      Clone3F3-FMA
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3F3-FMA is a mouse monoclonal antibody that detects human Transferrin receptor protein 1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tfrc
      • Trfr
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~90 kDa observed; 84.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-1080 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected TfR1 (CD71) in HT-1080 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1765-100UL 04065265944663

      Documentation

      Anti-TfR1 (CD71) Antibody, clone 3F3-FMA SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TfR1 (CD71) Antibody, clone 3F3-FMA Certificates of Analysis

      TitleLot Number
      Anti-TfR1 (CD71), clone 3F3-FMA - 3815820 3815820
      Anti-TfR1 (CD71), clone 3F3-FMA - 3821644 3821644
      Anti-TfR1 (CD71), clone 3F3-FMA - 3822341 3822341
      Anti-TfR1 (CD71), clone 3F3-FMA - 3884348 3884348
      Anti-TfR1 (CD71), clone 3F3-FMA - 3923511 3923511
      Anti-TfR1 (CD71), clone 3F3-FMA - 3939593 3939593
      Anti-TfR1 (CD71), clone 3F3-FMA - 3944557 3944557
      Anti-TfR1 (CD71), clone 3F3-FMA - 3959561 3959561
      Anti-TfR1 (CD71), clone 3F3-FMA - 3983452 3983452
      Anti-TfR1 (CD71), clone 3F3-FMA - 3995835 3995835
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