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577801 Anti-Tau Mouse mAb (TAU-5)

This Anti-Tau Mouse mAb (TAU-5) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tau.

Anti-Tau Mouse mAb (TAU-5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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577801
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, R, ShMMonoclonal Antibody

Pricing & Availability

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577801-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~45-68 kDa Tau protein.
      Catalogue Number577801
      Brand Family Calbiochem®
      Application Data
      Detection of human tau by immunoblotting. Samples: Whole cell lysate from SH-SY5Y neuronal cells. Primary antibody: Anti-Tau Mouse mAb (TAU-5) (Cat No. 577801) (1:1000). Detection: chemiluminescence.
      References
      ReferencesPapasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA 99, 1140.
      Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA 99, 6364.
      Product Information
      FormLiquid
      FormulationIn PBS.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (1-2 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (10 µg per 200-500 µg cell lysate)
      Paraffin Sections (1-2 µg/ml, heat pre-treatment required)
      Application CommentsWhen used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.
      Biological Information
      Immunogenpurified bovine microtubule-associated proteins
      ImmunogenBovine
      Epitopewithin the central region
      CloneTAU-5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is <50 µg/ml.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      577801-100UGCN 04055977265910

      Documentation

      Anti-Tau Mouse mAb (TAU-5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tau Mouse mAb (TAU-5) Certificates of Analysis

      TitleLot Number
      577801

      References

      Reference overview
      Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA 99, 1140.
      Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA 99, 6364.

      Citations

      Title
    • Hui, L., et al. 2012. Life Sci. 91, 1159.
    • Hui, L., et al. 2012. ASN Neuro. 4, 243.
    • Chen, X., et al. 2013. Neurobiol Aging 34, 2370.
      • .
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-May-2010 JSW
      ApplicationFrozen Sections (1-2 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (10 µg per 200-500 µg cell lysate)
      Paraffin Sections (1-2 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human tau by immunoblotting. Samples: Whole cell lysate from SH-SY5Y neuronal cells. Primary antibody: Anti-Tau Mouse mAb (TAU-5) (Cat No. 577801) (1:1000). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~45-68 kDa phosphorylated and unphosphorylated forms of tau.
      BackgroundTau is a neuronal microtubule-associated protein found predominantly in axons. Tau promotes tubulin polymerization and stabilizes microtubules, and it links certain signaling pathways to the cytoskeleton. Hyperphosphorylated tau is the major component of paired helical filaments, the building block of neurofibrillary lesions in Alzheimer’s disease. Hyperphosphorylation impairs the microtubule-binding function of tau, resulting in the destabilization of microtubules, and ultimately leading to degeneration of the affected neurons.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified bovine microtubule-associated proteins
      Epitopewithin the central region
      CloneTAU-5
      IsotypeIgG₁
      Specieshuman, mouse, rat, sheep
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsWhen used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is <50 µg/ml.
      Toxicity Standard Handling
      ReferencesPapasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA 99, 1140.
      Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA 99, 6364.
      Citation
    • Hui, L., et al. 2012. Life Sci. 91, 1159.
    • Hui, L., et al. 2012. ASN Neuro. 4, 243.
    • Chen, X., et al. 2013. Neurobiol Aging 34, 2370.
      • .