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MABC1794-100UG Anti-Sulf-2 Antibody, clone 5D5

Anti-Sulf-2, clone 5D5, Cat. No. MABC1794, is a mouse monoclonal antibody that detects Sulf-2 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.

MABC1794-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1794-100UG
      DescriptionAnti-Sulf-2 Antibody, clone 5D5
      Alternate Names
      • Extracellular sulfatase Sulf-2
      • hSulf-2
      • Arylsulfatase
      • EC:3.1.6.13
      • N-acetylglucosamine-6-sulfatase
      Background InformationExtracellular sulfatase Sulf-2 (UniProt: Q8IWU5; also known as hSulf-2, Arylsulfatase, EC:3.1.6.13, N-acetylglucosamine-6-sulfatase) is encoded by the SULF2 (also known as KIAA1247 and UNQ559/PRO1120) gene (Gene ID: 55959) in human. Sulfatase 1 (Sulf-1) and sulfatase 2 (Sulf-2) are heparan sulfatase editing sulfatases that remove 6-O sulfate moiety within heparan sulfate (HS) chains and alter the affinity of the HS chains for growth factors and growth factor receptors. Sulf-2 is synthesized with a signal peptide (aa 1-24) that is subsequently cleaved off to produce the mature protein. Its N-terminal region contains the catalytic domain (aa 1-415) that is followed by a hydrophilic domain (aa 416-715) and the C-terminal region. The hydrophilic domain is necessary for its endoglucosamine-6-sulfatase activity. Glycosylation at serine 583 is shown to negatively regulate its N-acetylglucosamine-6-sulfatase and arylsulfatase activities. Higher expression of Sulf-2 is reported in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney, and placenta. It is detected in the endoplasmic reticulum, Golgi apparatus, and on the cell surface. A secreted form has also been described that is produced after processing by furin. In a majority of hepatocellular carcinomas (HCC) Sulf-2 enhances tumor growth and progression via activation of the Wnt and TGF-β signaling. It has broad oncogenic effects in HCC, and it increase cell growth by upregulating glypican 3 that promotes release of FGF2 and Wnt form glypican 3 to bind to their cognate receptors and enhance receptor tyrosine kinase activity and Wnt/β-catenin signaling. Knockout of Sulf-2 is reported to suppress HCC tumorigenesis. Two isoforms of Sulf-2 have been described that are produced by alternative splicing. (Ref.: Luo, X., et al. (2021). Hepatology. 74(3); 1411-1428; Singer, MS., et al. (2015). Clin. Chim. Acta. 440; 72-78).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Sulf-2, clone 5D5, Cat. No. MABC1794, is a mouse monoclonal antibody that detects Sulf-2 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Inhibits Activity/Function
      • ELISA
      Application NotesTested Applications

      Inhibition Assay: A representative lot of this antibody inhibited the PDGFRβ-YAP pathway and cholangiocarcinoma (CCA) growth in a murine model. Luo, X., et al. (2021). Hepatology.;74(3):1411-1428.

      Western Blotting Analysis: A representative lot detected Sulf-2 in Western Blotting applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).

      Immunoprecipitation Analysis: A representative lot detected Sulf-2 in Immunoprecipitation applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).

      ELISA: A representative lot detected Sulf-2 in ELISA applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenFull-length recombinant human Sulf-2.
      EpitopeUnknown
      Clone5D5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5D5 is a mouse monoclonal antibody that detects Sulf-2.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SULF2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~66 kDa for His/GST-tagged recombinant fragment of human Sulf-2; 100.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with His/GST-tagged recombinant Human sulfatase 2 (Sulf-2).

      Western Blotting Analysis: A 1:250 dilution of this antibody detected His/GST-tagged recombinant Human sulfatase 2 (Sulf-2).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1794-100UG 04065270321695