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MABS2179-100UG Anti-Phosphotyrosine (Fab fragment) Antibody, clone 4G10-G6

MABS2179-100UG
100 μg  
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      Overview

      Description
      Catalogue NumberMABS2179-100UG
      DescriptionAnti-Phosphotyrosine (Fab fragment) Antibody, clone 4G10-G6
      Alternate Names
      • p-Tyrosine
      • pY
      Background InformationPhosphorylation on tyrosine residues is one of the major signal transduction mechanism that regulates intercellular and intracellular signaling in multicellular organisms. Dysregulation of protein tyrosine phosphorylation leads to several diseases, including cancer, diabetes, and several neurodegenerative disorders. Tyrosine residues in proteins can be phosphorylated by protein tyrosine kinases resulting in the formation of phosphotyrosine. Tyrosine phosphorylation is one of the key steps in signal transduction and regulation of enzymatic activity. Anti-phosphotyrosine antibodies are commonly used in Western blotting application after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. They can also be used directly in cell lysates to examine the overall change of tyrosine phosphorylation level in response to various hormones, cytokines, and other treatments. Clone 4G10® is one of the best monoclonal antibodies for the detection tyrosine phosphorylation. This clone provides high sensitivity and can detect tyrosine phosphorylation of multiple proteins. It interacts only with the phosphotyrosine (pY) residue and not with any neighboring ones. Clone 4G10-G6 is recombinantly expressed using E. coli in a Fab format and offers about 7-fold improved signal over parental 4G10. It allows for the detection of difficult to detect bands, particularly in the range of 25 to 55 kDa when used with anti-His Tag secondary antibody. (Ref.: Mou, Y., et al. (2018). J. Am. Chem. Soc. 140, 16615-16624.
      Product Information
      FormatPurified
      PresentationRecombinant monoclonal antibody Fab in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Phosphotyrosine (Fab fragment), clone 4G10-G6 Cat. No. MABS2179, is a recombinant monoclonal antibody that detects phosphotyrosines in protein and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected Phosphotyrosine in HeLa cells treated with 0.1 mM pervanadate. (Mou, Y., et al. (2018). J. Am. Chem. Soc. 140(48); 16615-16624).

      Immunohistochemistry (Paraffin) Analysis (IHC(P)): 3 µg/mL of this antibody detected Phosphotyrosine in human breast cancer tissue sections using an anti-His Tag secondary antibody and HRP-DAB detection system.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant antibody from Fabs constructed in a dual expression vector for Light chain and His-tagged heavy chain of clone 4G10 and expressed in C43(DE3) strain of E. coli.
      EpitopePhosphotyrosine
      Clone4G10-G6
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostE. coli
      SpecificityClone 4G10-G6 is a his tagged recombinant monoclonal antibody that detects phosphotyrosine in proteins from all species. To be used with anti-His Tag secondary antibody.
      Species Reactivity
      • All
      Species Reactivity NotePredicted to react with all species.
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodImmobilized Metal Affinity Chromatography (IMAC)
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from Jurkat cells treated with pervanadate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Phosphotyrosine in lysate of Jurkat cells treated with 0.1 mM pervanadate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2179-100UG 04065265237840