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MAB5272 Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324

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MAB5272
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, IHC, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5272
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
      Alternate Names
      • CD171
      • N-CAM L1
      Background InformationFamilies of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationRat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blotting:
      20 μg/mL was used on a previous lot.

      Immunocytochemistry:
      5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.

      Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
      Clone324
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityMonoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
      Gene Symbol
      • VCAM1
      • INCAM-100
      • MGC99561
      • CD106
      • DKFZp779G2333
      • L1CAM
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
      SIZE: 739 amino acids; 81276 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
      DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
      PTM: Sialoglycoprotein.
      DISEASE: SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
      SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Molecular Weight120-140 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5272 04053252502118