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ABC10 Anti-Megf10 Antibody

Anti-Megf10, Cat. No. ABC10, is a rabbit polyclonal antibody that detects Multiple epidermal growth factor-like domains protein 10 (Mefg10) and is tested for use in Immunofluorescence and Western Blotting.

Anti-Megf10 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Megf10 UniProt Number: Q6DIB5
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ABC10
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC10
      DescriptionAnti-Megf10 Antibody
      Alternate Names
      • Multiple epidermal growth factor-like domains protein 10
      • Multiple EGF-like domains protein 10
      Background InformationMultiple epidermal growth factor-like domains protein 10 (UniProt; Q6DIB5; also known as Multiple EGF-like domains protein 10, Megf10) is encoded by the Megf10 gene (Gene ID: 70417) in murine species. Megf10 is a type I transmembrane membrane receptor protein that is involved in phagocytosis by macrophages and astrocytes of apoptotic cells. Megf10 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 26-857), a transmembrane protein (aa 858-878), and a cytoplasmic domain (aa 879-1147). It also contains 15 EGF-like domains in the extracellular domain. Its expression is observed in developing myoblasts, muscle stellate cells, stellate cells of the cerebellum, kidney, and macrophages. It is expressed at the cell surface in clusters around cell corpses during engulfment. It serves as a receptor for C1q that binds phosphatidylserine expressed on the surface of apoptotic cells. It is shown to be essential for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum. Megf10 also plays a role in muscle cell proliferation, adhesion, and motility and is shown to be an essential factor in the regulation of myogenesis. It controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway. Mutations in Megf10 gene are reported to cause early onset myopathy, areflexia, respiratory distress, and dysphagia. (Ref.: Saha, M., et al. (2107). Human Mol. Gen. 15(1); 2984-3000).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Mouse retina tissue lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Megf10, Cat. No. ABC10, is a rabbit polyclonal antibody that detects Multiple epidermal growth factor-like domains protein 10 (Mefg10) and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A representative lot was used to detect Megf10 in P7 caudal brain tissue and retina cells by an independent laboratory. (Image courtesy form the laboratory of Dr. Joshua Sanes, Harvard University).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the C-terminal, cytoplasmic domain of murine multiple epidermal growth factor-like domains protein 10 (Megf10).
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects multiple epidermal growth factor-like domains protein 10 (Megf10). It targets an epitope within the cytoplasmic domain.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Megf10
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~130 kDa observed; 122.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse Brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Megf10 in Mouse Brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC10 04053252550188

      Documentation

      Anti-Megf10 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Megf10 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Megf10 2480948
      Anti-Megf10 (rabbit polyclonal) - 3062301 3062301
      Anti-Megf10 (rabbit polyclonal) - 3523424 3523424
      Anti-Megf10 (rabbit polyclonal) - 3523940 3523940
      Anti-Megf10 (rabbit polyclonal) - 3677727 3677727
      Anti-Megf10 (rabbit polyclonal) - 3755443 3755443
      Anti-Megf10 (rabbit polyclonal) - 3821720 3821720
      Anti-Megf10 (rabbit polyclonal) - 3841549 3841549
      Anti-Megf10 (rabbit polyclonal) - 3852322 3852322
      Anti-Megf10 (rabbit polyclonal) - 3892225 3892225
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      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies