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09-864 Anti-JHDM1B Antibody

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09-864
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-864
      DescriptionAnti-JHDM1B Antibody
      Alternate Names
      • JMJD
      • lysine (K)-specific demethylase 2B
      • F-box protein FBL10
      • [Histone-H3]-lysine-36 demethylase 1B
      • JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein
      • F-box and leucine-rich repeat protein 10
      • protein containing CXXC domain 2
      • jumonji C domain-containing histone demethylase 1B
      • Jumonji domain-containing EMSY-interactor methyltransferase motif protein
      • lysine-specific demethylase 2B
      • Protein-containing CXXC domain 2
      • JmjC domain-containing histone demethylation protein 1B
      • Protein JEMMA
      • F-box/LRR-repeat protein 10
      • CXXC-type zinc finger protein 2
      Background InformationJmjC domain-containing histone demethylation protein 1B, also known as Lysine-specific demethylase 2B, is a nuclear enzyme encoded by the JHDM1B gene (also known as KDM2B) in human. JHDM1A/KDM2B exhibits H3K36-specific demethylase activity with a preference for H3K36me2 over H3K36me and H3K36me3 (PMID 16362057). JHDM1A/KDM2B is also reported to negatively regulate cell proliferation by targeting rDNA and thereby repressing the transcription of rRNA in a JmjC-domain-dependent manner (PMID 17994099).
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-JHDM1B Antibody is a rabbit polyclonal antibody for detection of JHDM1B also known as lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected JHDM1B in NIH/3T3 and HeLa cells.
      Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JHDM1B in A431 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JHDM1B.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Rabbit, Chimpanzee, Horse, and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq].
      Gene Symbol
      • KDM2B
      • CXXC2
      • FBXL10
      • JHDM1B
      • PCCX2
      • Fbl10
      • FBL10
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~165 kDa observed. An uncharacterized lower molecular weight band may be observed in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 1 µg/mL of this antibody detected JHDM1B on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      09-864 04053252415302