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AB1574-I-25UG Anti-GABA Transporter-3 Antibody, CT

Anti-GABA Transporter-3, CT, Cat. No. AB1574-I, is a rabbit polyclonal antibody that detects Sodium- and chloride-dependent GABA transporter 3 and is tested in Immunohistochemistry (Paraffin) and Western Blotting.

AB1574-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAB1574-I-25UG
      DescriptionAnti-GABA Transporter-3 Antibody, CT
      Alternate Names
      • Sodium- and chloride-dependent GABA transporter 3
      • GAT-3
      • Solute carrier family 6 member 11
      Background InformationSodium- and chloride-dependent GABA transporter 3 (UniProt: P31647; also known as GAT-3, Solute carrier family 6 member 11, GABA Transporter 3) is encoded by the Slc6a11 (also known as Gabt3, Gat-3, Gat-b) gene (Gene ID: 79213) in rat. GABA is reported to be the main inhibitory neurotransmitter in the mammalian brain, particularly in the basal ganglia network. Following its release from the presynaptic terminal, it is rapidly cleared from the extracellular space by GABA transporters (GATs) resulting it s the termination of inhibitory synaptic transmission. Thus far, four different GATs have been described in literature, GAT-1, GAT-2, GAT-3, and the Betain/GABA transporter type 1. GAT-3 is a multi-pass membrane protein that has one extracellular domain (aa 147-220), two cytoplasmic domains (aa 1-53 and 567-627), and twelve helical domains. It is shown to be highly expressed in the glomerular layer of the olfactory bulb, the inner nucleus of the retina, the thalamic paraventricular nucleus, and the globus pallidus. It terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. The GABA-transporting action of GATs is dependent on the Na+ gradient across the membrane. (Ref.: Jin XT., et al. (2011). Front. Syst. Neurosci. 5; 63).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GABA Transporter-3, CT, Cat. No. AB1574-I, is a rabbit polyclonal antibody that detects Sodium- and chloride-dependent GABA transporter 3 and is tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GABA Transporter-3 in rat brain tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal, cytoplasmic domain of rat GABA Transporter 3.
      EpitopeC-terminus
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects GABA Transporter 3. It targets an epitope within 21 amino acids from the C-terminal, cytoplasmic domain.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc6a11
      • Gabt3
      • Gat-3
      • Gat-b
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~59 Da observed; 69.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat Brain Microsomal Prep.

      Western Blotting Analysis: 1 µg/mL of this antibody detected GABA Transporter-3 in Rat brain microsomal preparations.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB1574-I-25UG 04061842196326