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DR1061 Anti-ENO1 Mouse mAb (8G8)

Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).

Anti-ENO1 Mouse mAb (8G8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-α Enolase 1

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DR1061
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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DR1061-100UGCN
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      Description
      OverviewRecognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.
      Catalogue NumberDR1061
      Brand Family Calbiochem®
      SynonymsAnti-α Enolase 1
      Application Data
      Detection of human ENO1 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1-5 µg/ml). Detection: chmiluminescence.

      Detection of human ENO1 by Immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (10 µg/ml). Detection: fluorescence.

      Detection of human ENO1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lymphoma tissue. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesHo, J.A., et al. 2010. Anal. Chem. 82 5944.
      Zhao, J., et al. 2007. Mol. Cell Proteomics. 6 987.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.30.
      Positive controlMCF-7 cells, Human lymphoma tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
      Clone8G8
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1061-100UGCN 04055977226027

      Documentation

      Anti-ENO1 Mouse mAb (8G8) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Ho, J.A., et al. 2010. Anal. Chem. 82 5944.
      Zhao, J., et al. 2007. Mol. Cell Proteomics. 6 987.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-α Enolase 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application Data
      Detection of human ENO1 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1-5 µg/ml). Detection: chmiluminescence.

      Detection of human ENO1 by Immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (10 µg/ml). Detection: fluorescence.

      Detection of human ENO1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lymphoma tissue. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.
      BackgroundThis protein is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
      HostMouse
      ImmunogenFull-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
      Clone8G8
      IsotypeIgG₁
      Specieshuman
      Positive controlMCF-7 cells, Human lymphoma tissue, HeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.30.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesHo, J.A., et al. 2010. Anal. Chem. 82 5944.
      Zhao, J., et al. 2007. Mol. Cell Proteomics. 6 987.