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MAB3234 Anti-Cytokeratin 18 Antibody, clone RGE53

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MAB3234
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, Ch, H, Ht, M, Po, R, RbFC, ICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3234
      Replaces04-586
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 18 Antibody, clone RGE53
      Background InformationCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
      Product Information
      FormatPurified
      Control
      • Skin , Pancreas/tumor
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytokeratin 18 Antibody, clone RGE53 is an antibody against Cytokeratin 18 for use in FC, IC, IH & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot

      Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.

      Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.

      Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCytoskeletal preparation from HeLa cells
      CloneRGE53
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecifically recognizes glandular epithelial cells from gastro-intestinal tract, respiratory tract and urogenetical tract. As well as endocrine and exocrine tissues and mesothelial cells. It is unreactive with stratified squamous epithelia (esophagus, epidermis, ectocervix). In transitional epithelium of the bladder RGE53 only reacts with the superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumours. The antibody is directed against 45 kD cytokeratin protein (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Chicken
      • Human
      • Hamster
      • Mouse
      • Pig
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryKRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • KRT18
      • K18
      • Keratin-18
      • CYK18
      • Cytokeratin-18
      • CK-18
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05783 # Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
      SIZE: 430 amino acids; 48058 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-18 associates with keratin-8. Interacts with the thrombin- antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.
      SUBCELLULAR LOCATION: Cytoplasm, perinuclear region.
      TISSUE SPECIFICITY: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
      PTM: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. & Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.DISEASE:SwissProt: P05783 # Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].
      SIMILARITY: SwissProt: P05783 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Molecular Weight45 kD
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3234 04053252667343