Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 is validated for use in Immunocytochemistry, Flow Cytometry, Immunohistochemistry for the detection of Active-β-Catenin.
More>>This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 is validated for use in Immunocytochemistry, Flow Cytometry, Immunohistochemistry for the detection of Active-β-Catenin. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
References
Product Information
Format
AlexaFluor®488
Presentation
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 is validated for use in Immunocytochemistry, Flow Cytometry, Immunohistochemistry for the detection of Active-β-Catenin.
Key Applications
Immunocytochemistry
Flow Cytometry
Immunohistochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in A431 cells. Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected Active-β-Catenin in 1X10E6 A431 and HeLa cells. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Active-β-Catenin in human colon and human colorectal adenocarcinoma tissue. The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).
Biological Information
Immunogen
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
Epitope
The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
Clone
8E7
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with Rat, Zebrafish based on 100% sequence homology. The antibody eptiope is 100% conserved in zebrafish. .
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
