Chromolith® HPLC Columns Speed of Analysis

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Chromolith® HPLC columns owe their rapid separation speed to their unique bimodal pore structure with macro- and mesopores. The macropores reduce the column back pressure and allow the use of faster flow rates, thereby considerably reducing analysis time. Whereas, the mesopores form a fine porous structure, which creates a very large active surface area for high-efficiency separations.

This combination results in separations which are twice as fast as conventional 5 μm particulate columns – at half the back pressure. Furthermore, flow rates can be easily varied from 1 mL up to 9 mL per minute, with the same high-quality resolution. Besides rapid separations, Chromolith® HPLC columns also ensure fast re-equilibration between analyses.

High-Speed Separation of Five Beta-Blockers

A mixture of five beta-blocker drugs demonstrates the extreme time savings and high separation efficiency made possible with Chromolith® columns. Due to the excellent mass transfer properties of the monolithic skeleton, high-speed separation is possible even at a high flow rate. The beta-blockers were well separated with excellent peak symmetry. At 9 mL/min, the analysis time was less than one minute, and the column back pressure was only 153 bar.

Application Example

Column	Chromolith® Performance RP-18 endcapped, 100-4.6 mm
Mobile phase	Isocratic acetonitrile / 0.1% trifluoroacetic acid in water, 20/80 (v/v)
Pressure	Total pressure (including HPLC system) 25°C
Detection	UV 220 nm
Injection volume	5 μL
Sample	Atenolol 63 μg/mL Pindolol 29 μg/mL Metoprolol 108 μg/mL Celiprolol 104 μg/mL Bisoprolol 208 μg/mL