Chromolith® HighResolution RP-18 Endcapped Flow Rate |
Compared to core-shell material, the faster flow rates of Chromolith® HighResolution columns considerably reduce analysis time while extending column lifetime. At a flow rate of 1 ml/min, a chromatogram run on a Chromolith® HighResolution HPLC column looks almost identical to the same chromatogram run on a particulate column packed with sub 3 µm particles. Likewise, Chromolith® HighResolution is able to generate similar results as a column packed with 2.6 µm ID core-shell particles, but at much lower back pressures.
Chromolith® HighResolution RP-18e |
Ordering Number |
---|---|
100 mm | 152022 |
50 mm | 152021 |
25 mm | 152020 |
5 mm Guard Columns (3 pieces) | 152025 |
Silica monolithic
|
Core-shell
|
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Silica monolithic: 40 bar (Inj. volume) | Core-shell: 100 bar (Inj. volume) |
Mobile phase | A: Acetonitrile B: 20 mM phosphate buffer pH 4.5 |
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Gradient | Time/min | %A | %B |
0.0 | 20 | 80 | |
12.0 | 40 | 60 | |
Flow rate | 1.0 ml/min | ||
Detection | UV 254 nm | ||
Temperature | 22°C | ||
Inj. volume | 2 µl | ||
Sample | 1. Ascorbic acid 2. 4-Hydroxybenzoic acid 3. Sorbic acid 5. Methyl 4-hydroxybenzoate 6. Ethyl 4-hydroxybenzonate 7. Propyl 4-hydroxybenzonate |