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71110 rLysozyme™ Solution

Stabilized recombinant lysozyme

rLysozyme™ Solution: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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71110
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概要

Replacement Information

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71110-3CN
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      		 樹脂ｱﾝﾌﾟﾙ 300 ku
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      71110-4CN
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          		 樹脂ｱﾝﾌﾟﾙ 1200 ku
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          71110-5CN
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              		 樹脂ｱﾝﾌﾟﾙ 6000 ku
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              Description
              OverviewrLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
              Catalogue Number71110
              Brand Family Novagen®
              References
              Product Information
              Unit of DefinitionOne unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.
              Quality LevelMQ400
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Standard Handling
              Storage -20°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              カタログ番号 GTIN
              71110-3CN 07790788055547
              71110-4CN 04055977254617
              71110-5CN 07790788053048

              Documentation

              rLysozyme™ Solution (M)SDS

              タイトル

              英語版製品安全データシート（(M)SDS） 

              rLysozyme™ Solution 試験成績書（CoA）

              タイトルロット番号
              71110

              カタログ

              タイトル
              Bulk Product Guide
              Protein Purification and Detection Tools
              安定したタンパク質抽出と効率的なサンプル調整に　Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品

              技術情報

              タイトル
              Benzonase®endonuclease for improved primary recovery in an E. coli-based process for Fab production
              TB334 rLysozyme Solution

              引用

              Title
            • Rachel Balder, et al. (2007) Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Infection and Immunity 75, 2765-2775.
            • Benedict M. Long, et al. (2007) Analysis of carboxysomes from Synechococcus PCC7942 reveals multiple rubisco complexes with carboxysomal proteins CcmM and CcaA. Journal of Biological Chemistry 282, 29323-29335.
            • Sehyung Cho, et al. (2005) Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and ubc9. Molecular Endocrinology 19, 290-311.
            • S. Moy, et al. (2004) Genome-scale expresion of proteins from Bacillus subtilis. Journal of Structural and Functional Genomics 5, 103-109.
            • Mark A. Rizzo, et al. (2004) An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnology 22, 445-449.
            • B. Husen, et al. (2003) Characterization of 17β-hydroxysteroid dehygronase type 7 in reproductive tissues of the marmoset monkey. Biology of Reproduction 68, 2092-2099.
              		•

              ユーザープロトコール

              タイトル
              TB334 rLysozyme™ Solution