71339 Sigma-AldrichpIEx™-7 Ek/LIC Vector Kit - Novagen
Novagen's pIEx-7 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus.
More>> Novagen's pIEx-7 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. Less<<pIEx™-7 Ek/LIC Vector Kit - Novagen: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
同義語: InsectDirect™ pIEx™-7 Ek/LIC Vector Kit
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概要
| Replacement Information |
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価格＆在庫状況
| カタログ番号 | 在庫状況 | 包装 | Qty/Pk | 価格 | 数量 | |
|---|---|---|---|---|---|---|
| 71339-3CN |
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樹脂ｱﾝﾌﾟﾙ | 20 rxn |
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| Description | |
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| Overview | The pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1). |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 71339 |
| Brand Family | Novagen® |
| Synonyms | InsectDirect™ pIEx™-7 Ek/LIC Vector Kit |
| References | |
|---|---|
| References | Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
| Product Information | ||
|---|---|---|
| • | 1 µg | Ek/LIC Vector |
| • | 25 units | T4 DNA Polymerase, LIC-qualified |
| • | 50 µl | 25 mM EDTA |
| • | 40 µl | 25 mM dATP |
| • | 8 µl | Ek/LIC β-gal Control Insert |
| • | 100 µl | 100 mM DTT |
| • | 50 µl | 10X T4 DNA Polymerase Buffer |
| • | 1.5 ml | Nuclease-free Water |
| • | 5 × 2 ml | SOC Medium |
| • | 10 µl | Test Plasmid |
| • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells |
| Quality Level | MQ100 | |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| カタログ番号 | GTIN |
| 71339-3CN | 04055977253269 |
Documentation
pIEx™-7 Ek/LIC Vector Kit - Novagen 試験成績書（CoA）
| タイトル | ロット番号 |
|---|---|
| 71339 |
参考資料
| 参考資料の概要 |
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| Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
カタログ
| タイトル |
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| Insect Cell Expression |
| Insect Directﾙ Protein Expression & Purification System |
| Radiance! Multisystem Protein Expression |
引用
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ユーザープロトコール
| タイトル |
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| TB163 Ek/LIC Cloning Kits |
ベクターマップ
| タイトル |
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| TB399VM pIEx-7 Ek/LIC Vector Map |
ベクター配列
| タイトル |
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| pIEx-7 Ek/LIC Vector Sequence |