71333 Sigma-AldrichpIEx™-6 DNA - Novagen
Novagen's pIEx™-6 vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus.
More>> Novagen's pIEx™-6 vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. Less<<pIEx™-6 DNA - Novagen: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
同義語: InsectDirect™ System- pIEx™-6 DNA
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概要
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価格＆在庫状況
| カタログ番号 | 在庫状況 | 包装 | Qty/Pk | 価格 | 数量 | |
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| 71333-3CN |
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樹脂ｱﾝﾌﾟﾙ | 20 μg |
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| Description | |
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| Overview | The pIEx™-6 vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. This plasmid features an amino-terminal His•Tag® coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector-encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional S•Tag™coding sequence is present at the distal end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (1). |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 71333 |
| Brand Family | Novagen® |
| Synonyms | InsectDirect™ System- pIEx™-6 DNA |
| References | |
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| References | 1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
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| Quality Level | MQ100 |
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| Ship Code | Shipped with Blue Ice or with Dry Ice |
| Toxicity | Standard Handling |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Global Trade Item Number | |
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| カタログ番号 | GTIN |
| 71333-3CN | 04055977253238 |
Documentation
pIEx™-6 DNA - Novagen (M)SDS
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pIEx™-6 DNA - Novagen 試験成績書（CoA）
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| 71333 |
参考資料
| 参考資料の概要 |
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| 1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
カタログ
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| Insect Cell Expression |
| Insect Directﾙ Protein Expression & Purification System |
ユーザープロトコール
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| TB356 InsectDirect™ pIEx™ Vectors |
ベクターマップ
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| TB398 pIEx™-6 Vector |
ベクター配列
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| pIEx-6 Vector Sequence |