Millipore Sigma Vibrant Logo

71340 pCDFDuet™-1 DNA - Novagen

pCDFDuet™-1 DNA - Novagen: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
View This Product on Sigma-Aldrich
71340
価格＆在庫状況を照会

概要

Replacement Information

価格＆在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
71340-3CN
在庫状況検索中…
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      		 樹脂ｱﾝﾌﾟﾙ 10 μg
      価格を検索中…
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示　
      値引
      ()
       
      —
      弊社照会
      		 —
      Description
      OverviewpCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number71340
      Brand Family Novagen®
      Application Data

      LaneM: Trail Mix™ Protein Markers; Lane1: pACYDuet-1:β-gal + Fluc; Lane2: pETDuet-1:GST/GUS + GFP; Lane3: pRSFDuet-1:Nus/hlFNγ + S•Tag/T4 PNK; Lane4: pCDFDuet-1:GUS + His•Tag/MBP; Lane5: pACYDuet-1, pETDuet-1 combination; Lane6: pACYDuet-1, pRSFDuet-1 combination; Lane7: pACYDuet-1, pCDFDuet-1 combination; Lane8: pETDuet-1, pRSFDuet-1 combination; Lane9: pRSFDuet-1, pCDFDuet-1 combination; Lane10: pETDuet-1, pCDFDuet-1 combination; Lane11: pACYDuet-1, pETDuet-1, pRSFDuet-1 combination; Lane12: pACYDuet-1, pETDuet-1, pCDFDuet-1 combination; Lane13: pACYDuet-1, pRSFDuet-1, pCDFDuet-1 combination; Lane14: pETDuet-1, pRSFDuet-1, pCDFDuet-1 combination; Lane15: Uninduced control; Lane16: All four Duet vectors: all eight proteins

      The indicated constructs were transformed individually or together into BL21(DE3). Cultures were grown in TB plus phosphates plus glucose at 37°C to and Abs600 between 1.0 and 1.2. Target protein expression was induced by adding IPTG to a final concentration of 1 mM. Cultures were harvested by centrifugation 2.5 h after induction. Lysates were produced by sonication using equal volumes of 1% SDS and 2X SDS sample buffer. Equivalent amounts of protein (based on harvest ABS) were analyzed by SDS-PAGE (4-20% gradient gel) and stained with coomassie blue. To maximize band separation, proteins smaller than 25 kDa were allowed to migrate off the gel.
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      71340-3CN 04055977253276

      Documentation

      pCDFDuet™-1 DNA - Novagen (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      pCDFDuet™-1 DNA - Novagen 試験成績書（CoA）

      タイトルロット番号
      71340

      引用

      Title
    • Louis B. Rice, et al. (2007) Interaction of related Tn916-like transposons: analysis of excision events promoted by Tn916 and Tn5386 integrases. Journal of Bacteriology 189, 3909-3917.
    • Mary Anne T. Rubio, et al. (2007) An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Procedings of the National Academy of Science 104, 7821-7826.
    • Kai-Hong Zhao, et al. (2007) Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Procedings of the National Academy of Science 104, 14300-14305.
    • Wonduck Kim and F. Robert Tabita. (2006) Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. Journal of Bacteriology 188, 6544-6552.
    • Zhizhong Li, et al. (2006) Structure of a BMI-1-ring1B polycomb group ubiquitin ligase complex. Journal of Biological Chemistry 281, 20643-20649.
    • Niraj H. Tolia and Leemor Joshua-Tor. (2006) Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55-64.
    • Matthew A. Young, et al. (2006) Structure of the kinase domain of an imatinib-resistant Abl mutant in complex with the aurora kinase inhibitor VX-680. Cancer Research 66, 1007-1014.
    • Kai-Hong Zhao, et al. (2006) Chromophore attachment to phycobiliprotein -subunits: phycocyanobilin:cysteine-84 phycobiliprotein lyase activity of Cpes-like protein from Anabaena SP. PCC7120. Journal of Biological Chemistry 281, 8573-8581.
    • Melissa L. Geddie, et al. (2005) Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. Journal of Biological Chemistry 280, 35641-35646.
    • Markus Raschle, et al. (2004) Multiple interactions with the Rad51 recombinase govern the homologous recombination function of Rad54. Journal of Biological Chemistry (sept 2004) e copy,.
      		•

      ユーザープロトコール

      タイトル
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB340 Duet Vectors
      TB401 pRSF and pCDF Vectors

      ベクターマップ

      タイトル
      TB390VM pCDFDuet™-1 Vector Map

      ベクター配列

      タイトル
      pCDFDuet™-1 Vector Sequence