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			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

164610 Sigma-Aldrichp-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem

An organomercurial agent used for the activation of latent MMPs in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.

p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: APMA

Primary Target: Activation of latent MMPs in vitro

View This Product on Sigma-Aldrich

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概要

Replacement Information

主要スペック表

CAS #	Empirical Formula
6283-24-5	C₈H₉HgNO₂

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
164610-700MGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	Plastic Bag(s)	700 mg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	An organomercurial agent used for the activation of latent MMPs in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
Overview	Note: 1 set = 5 x 700 mg.
Catalogue Number	164610
Brand Family	Calbiochem®
Synonyms	APMA

References
References	Chen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113. Marcy, A.I., et al. 1991. Biochemistry 30, 6476. Stricklin, G.P., et al. 1983. Biochemistry 22, 61.

Product Information
CAS number	6283-24-5
ATP Competitive	N
Form	Yellow solid
Hill Formula	C₈H₉HgNO₂
Chemical formula	C₈H₉HgNO₂
Reversible	N
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Primary Target	Activation of latent MMPs in vitro
Purity	≥90% by HPLC

Physicochemical Information
Cell permeable	N

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	OV5550000

Safety Information
R Phrase	R: 26/27/28-36/37/38 Very toxic by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin.
S Phrase	S: 36 Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Highly Toxic
Hazardous Materials Attention:	Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage	+15°C to +30°C
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
164610-700MGCN	04055977207637

Documentation

p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem 試験成績書（CoA）

タイトル	ロット番号
164610	ロット番号を入力

参考資料

参考資料の概要
Chen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113. Marcy, A.I., et al. 1991. Biochemistry 30, 6476. Stricklin, G.P., et al. 1983. Biochemistry 22, 61.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	12-February-2024 JSW
Synonyms	APMA
Description	An organomercurial agent used for the activation of latent matrix metalloproteinases (MMPs) in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
Form	Yellow solid
Recommended reaction conditions	The MMPs are members of a family of at least twenty Zn²⁺-dependent endopeptidases that function extracellularly at neutral pH. The MMPs are initially synthesized as latent or inactive zymogens that contain a regulatory propeptide. This propeptide confers the latency by binding to the Zn²⁺ at the enzyme's active site. MMP activation can be achieved by autoactivation and by the action of other MMPs or proteases, such as furin, urokinase, plasmin, and trypsin, as well as by organomercurial compounds. The following is a protocol from Stricklin, et al. (1983. Biochemistry 22, 61) describing the use of paminophenylmercuric acetate (APMA) to activate pro-MMPs. This protocol is good for other types of organomercurials such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), and mersalyl. Organomercurial stock solutions Prepare stock solutions in 0.1 M NaOH at a concentration range of 10-50 mM just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl. (e.g. Marcy, A.I. et al. 1991. Biochemistry 30, 6476). ProMMPs Prepare proenzyme solutions in 0.1 M Tris-HCl, pH 7.5 at 1-2 mg/ml. Activation of proMMPs To initiate activation, combine proMMP solution with organomercurial stock in a 10:1 volume* ratio (MMPs to organomercurial). Incubate the mixture at 37°C for 2-3 hrs*. When more organomercurial is desired, make the stock solution more concentrated. Do not exceed the above ratio so that the volume of organomercurial in the mixture is more than about 1/10 of that of the proMMP solution; this could result in significant changes in pH. It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with fixed concentration of proMMP and organomercurial would be incubated as described above. Remove aliquots of sample at various time points. Stop the reaction by the addition of concentrated SDS-sample buffer (for example, add 10 µl of 2X sample buffer to a 10 µl aliquot or add 2 µl of 5X sample buffer to an 8 µl aliquot) and then boil. The progress of the activation can then be monitored qualitatively by analyzing these time aliquots on a 12% SDS-PAGE gel. Removing organomercurial from the mixture after activation** The activated MMPs can be used without the removal of the organomercurial. Researchers are referred to the following reference for the removal of organomercurial by gel filtration if desired: Marcy, A.I., et al. 1991. Biochemistry 30, 6476
CAS number	6283-24-5
RTECS	OV5550000
Chemical formula	C₈H₉HgNO₂
Structure formula
Purity	≥90% by HPLC
Solubility	DMSO (10 mg/ml) or 0.1 N NaOH (10 mg/ml)
Storage	+15°C to +30°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
Toxicity	Highly Toxic
References	Chen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113. Marcy, A.I., et al. 1991. Biochemistry 30, 6476. Stricklin, G.P., et al. 1983. Biochemistry 22, 61.

カテゴリー

Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Activators > MMP Activators

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