480716
α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli
別名:
α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, N-Acetylneuraminyl Hydrolase, Sialidase
recombinant
expressed in E. coli
description
RTECS - QQ3450000
form
liquid
specific activity
≥40 units/mg protein
≥5 units/mL
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Harmful (C)
General description
Note: 1 mU = 1 milliunit.
Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
Other Notes
Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
Physical form
In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Sarah W Nelson et al.
Influenza and other respiratory viruses, 13(6), 593-602 (2019-08-09)
The influenza A virus (IAV) binds to α-2,3- and α-2,6-linked sialic acid (SA) receptors expressed by Madin-Darby canine kidney (MDCK) cells. The receptor distribution may therefore be important in regulating IAV propagation. Serum-free medium (SFM) avoids variability in conventional culture
Karima S Khimani et al.
Scientific reports, 10(1), 2933-2933 (2020-02-23)
To compare goblet cell (GC) number and area in the covered superior (SB) versus exposed temporal (TB) bulbar conjunctiva in control versus aqueous tear deficient eyes (ATD) and evaluate correlation with tear MUC5AC protein. SB and TB impression cytology performed
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 480716-250MIUCN | 04055977201437 |
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