420400
Anti-KDEL Mouse mAb (10C3)
liquid, clone 10C3, Calbiochem®
製品名
Anti-KDEL Mouse mAb (10C3), liquid, clone 10C3, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
10C3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (4 µg/mL, colorimetric)
Immunocytochemistry (5 µg/mL)
Immunoprecipitation (5 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Kdelr1(68137)
Analysis Note
Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
Application
Immunoblotting (4 µg/ml, colorimetric)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Disclaimer
Toxicity: Highly Toxic (H)
General description
Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Recognizes the Grp78 (BiP) and Grp94 proteins.
This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.
Immunogen
Rat
a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
Other Notes
Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Smriti A Agrawal et al.
Human molecular genetics, 26(14), 2667-2677 (2017-05-06)
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Michael A Robichaux et al.
Disease models & mechanisms, 15(5) (2022-03-12)
The P23H mutation in rhodopsin (Rho), the rod visual pigment, is the most common allele associated with autosomal-dominant retinitis pigmentosa (adRP). The fate of misfolded mutant Rho in rod photoreceptors has yet to be elucidated. We generated a new mouse
Gary P Brennan et al.
PloS one, 8(1), e54491-e54491 (2013-01-30)
14-3-3 proteins are ubiquitous molecular chaperones that are abundantly expressed in the brain where they regulate cell functions including metabolism, the cell cycle and apoptosis. Brain levels of several 14-3-3 isoforms are altered in diseases of the nervous system, including
Vangelis Kondylis et al.
Autophagy, 9(6), 861-880 (2013-03-14)
Activation of TLR signaling has been shown to induce autophagy in antigen-presenting cells (APCs). Using high-resolution microscopy approaches, we show that in LPS-stimulated dendritic cells (DCs), autophagosomes emerge from MHC class II compartments (MIICs) and harbor both the molecular machinery
Hyojeong Hwang et al.
Cell reports, 41(11), 111802-111802 (2022-12-15)
Asymmetric localization of mRNAs is crucial for cell polarity and cell fate determination. By performing fractionation RNA-seq, we report here that a large number of maternal RNAs are associated with the ER in Xenopus oocytes but are released into the
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 420400-50UGCN | 04055977187571 |
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