Millipore Sigma Vibrant Logo

14-733 c-Kit (V654A) Protein, active, 10 µg

V654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.

c-Kit (V654A) Protein, active, 10 µg: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
View This Product on Sigma-Aldrich
14-733
10 µg  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

       

      お問合せください

      概要

      Replacement Information
      Description
      Catalogue Number14-733
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Descriptionc-Kit (V654A) Protein, active, 10 µg
      OverviewN-terminal GST tagged, recombinant human cKit, amino acids 544 - end containing the V654A mutation. Co-ordinates encompass the cytoplasmic domain (544-976) according in Swiss Prot P10721. V654A is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationV654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourcerecombinant protein expressed in Sf21 cells
      Specific Activity169 U/mg, where one unit of cKit (V654A) activity is defined as 1nmol phosphate incorporated into 250 μM GGMEDIYFEFMGGKKK per minute at 30 °C with a final ATP concentration of 100 μM.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • KIT
      • C-Kit
      • PBT
      • CD117
      • SCFR
      • c-kit
      Protein Targetc-Kit
      Purification MethodPurified using glutathione-agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
      SIZE: 976 amino acids; 109865 Da
      SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
      SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight76.7kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 250 μM GGMEDIYFEFMGGKKK.
      Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active cKit (V654A).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      14-733 04053252513480

      関連製品＆アプリケーション

      Alternative Packsize

      カタログ番号 説明  
      14-733M c-Kit (V654A) Protein, active, 250 µg 価格＆在庫状況を表示

      製品ファミリー

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
      詳細はこちら >>

      カテゴリー

      Life Science Research > タンパク質と酵素 > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases