数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

03-32-1501 Sigma-AldrichZ-Phe-Arg-7-amido-4-methylcoumarin, Hydrochloride - CAS 70382-26-2 - Calbiochem

Substrate for fluorogenic assay of plasma and glandular kallikreins.

Z-Phe-Arg-7-amido-4-methylcoumarin, Hydrochloride - CAS 70382-26-2 - Calbiochem: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
カタログ
Data Sheet

View This Product on Sigma-Aldrich

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概要

Replacement Information

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
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Description
Overview	Substrate for fluorogenic assay of plasma and glandular kallikreins. Also a substrate for cathepsin B, cathepsin L, and papain.
Catalogue Number	03-32-1501
Brand Family	Calbiochem®

References
References	Morita, T., et al. 1977. J. Biochem. 82, 1495.

Product Information
CAS number	70382-26-2
ATP Competitive	N
Form	White to off-white solid
Hill Formula	C₃₃H₃₆N₆O₆·HCl
Chemical formula	C₃₃H₃₆N₆O₆·HCl
Reversible	N
Quality Level	MQ200

Applications

Biological Information
Primary Target	Substrate for fluorogenic assay of plasma and glandular kallikreins
Purity	≥98% by HPLC

Physicochemical Information
Cell permeable	N
Peptide Sequence	Z-Phe-Arg-AMC, HCl

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Irritant
Storage	-20°C
Protect from Moisture	Protect from moisture
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
03-32-1501-25MGCN	04055977206760

Documentation

Z-Phe-Arg-7-amido-4-methylcoumarin, Hydrochloride - CAS 70382-26-2 - Calbiochem (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Z-Phe-Arg-7-amido-4-methylcoumarin, Hydrochloride - CAS 70382-26-2 - Calbiochem 試験成績書（CoA）

タイトル	ロット番号
03-32-1501	ロット番号を入力

参考資料

参考資料の概要
Morita, T., et al. 1977. J. Biochem. 82, 1495.

カタログ

タイトル
Cathepsins and Related Products Technical Bulletin

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	18-September-2008 RFH
Description	Substrate for fluorogenic assay of plasma and glandular kallikreins. Also serves as a substrate for cathepsin B, cathepsin L, and papain.
Form	White to off-white solid
Recommended reaction conditions	Protocol for the Assay of Cathepsin L Enzyme: Cathepsin L is one of the lysosomal cysteine proteinases involved in the turnover of endocytosed proteins. It also has a higher specific activity towards physiological substrates, such as collagen and fibronectin over other lysosomal enzymes. It has an acidic optimal pH of 5.5. It is rapidly inactivated, 85% loss of activity in ~15 min, at pH 7.0-7.4. Substrate: Z-Phe-Arg-7-amido-4-methylcoumarin is more sensitive to cathepsin L than cathepsin B, although both enzymes will cleave the substrate. To determine cathepsin L activity, the specific cathepsin B substrate, Z-Arg-Arg-7-amido-4-methylcoumarin (Cat. No. 03-32-1570), can be used. The substrate is non-fluorescent, but when hydrolyzed by the enzyme it releases highly fluorescent 7-amido-4-methylcoumarin (AMC). AMC can be quantitated with a spectrofluorometer using an excitation at ~370 nm and an emission at ~460 nm. Reagents: • Assay/Activator: 340 mM sodium acetate, 60 mM acetic acid, and 4 mM EDTA, Buffer pH 5.5; add DTT to a final concentration of 8 mM just prior to use. • Substrate: Prepare a 1 mM Z-Phe-Arg-7-amido-4-methylcoumarin stock solution in DMSO and store in a -20°C freezer. Dilute to 20 µM with H₂O prior to use. • Stop Solution: 100 mM sodium monochloroacetate, 70 mM acetic acid, and 30 mM sodium acetate, pH 4.3 • Diluent: BRIJ^® 35 (0.1% in H₂O) • Standard: Prepare a 1 mM AMC stock solution in DMSO and store in a 4°C refrigerator. Dilutions are made using a 1:1 mixture of Assay Buffer and Stop Solution. Procedure: 1. Using the Diluent, prepare 500 µl of enzyme sample in 5 ml glass or polystyrene tubes. 2. Add 250 µl of assay/activator buffer. Allow ~1 min. incubation at 30°C for temperature equilibration and enzyme activation. Note: longer incubation may result in loss of activity. 3. Add 250 µl of the 20 µM substrate solution and mix. 4. Following a 10 min incubation at 30°C, add 1 ml of Stop Solution. 5. Measure the fluorescence and determine the molar amount using a standard curve for AMC. The fluorescence of AMC is only minimally affected by pH in the range of 4-7.
CAS number	70382-26-2
Chemical formula	C₃₃H₃₆N₆O₆·HCl
Peptide Sequence	Z-Phe-Arg-AMC, HCl
Purity	≥98% by HPLC
Solubility	5% Acetic acid (1 mg/ml), H₂O (1 mg/ml), or DMSO (1 mg/ml)
Storage	Protect from moisture -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Irritant
References	Morita, T., et al. 1977. J. Biochem. 82, 1495.

カテゴリー

Life Science Research > タンパク質と酵素 > Substrate & Control Proteins > Protease Substrates

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