数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

504043 Sigma-AldrichRho Inhibitor II, Y16 - CAS 429653-73-6 - Calbiochem

Rho Inhibitor II, Y16 - CAS 429653-73-6 - Calbiochem: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: LARG Rho-Binding Domain Blocker, (E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenylpyrazolidine-3,5-dione, (4E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenyl-3,5-pyrazolidinedione, p115 Rho-Binding Domain Blocker, PDZ Rho-Binding Domain Blocker

Primary Target: RhoGEF

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概要

Replacement Information

主要スペック表

CAS #	Empirical Formula
429653-73-6	C₂₄H₂₀N₂O₃

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
5.04043.0001	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	ｶﾞﾗｽﾋﾞﾝ	10 mg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	A cell-permeable pyrazolidinedione compound that is shown to target RhoGEF DH-PH domain junction with high affinity (K_d = 65 nM) and effetively prevent RhoGEFs LARG, p115, and PDZ from interacting with RhoA, while displaying little potency against DBL-RhoA, LBC-RhoA, intersectin-Cdc42, or TrioN-Cdc42 interaction. Shown to completely prevent serum-induced activation of cellular RhoA, but not Cdc42 or Rac1, in NIH-3T3 cultures (10 µM) and RhoA downstream signaling events. Greatly synergizes with Rho GEF-binding domain blocker Rhosin (Cat. No. 555460) in blocking RhoA-LARG interaction and in preventing cellular RhoA activation (both drug at 5 µM).
Catalogue Number	504043
Brand Family	Calbiochem®
Synonyms	LARG Rho-Binding Domain Blocker, (E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenylpyrazolidine-3,5-dione, (4E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenyl-3,5-pyrazolidinedione, p115 Rho-Binding Domain Blocker, PDZ Rho-Binding Domain Blocker

References
References	Shang, X., et al. 2013. Proc. Natl. Acad. Sci. USA. 110, 3155.

Product Information
CAS number	429653-73-6
Form	Orange powder
Hill Formula	C₂₄H₂₀N₂O₃
Chemical formula	C₂₄H₂₀N₂O₃
Reversible	Y
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Primary Target	RhoGEF
Purity	≥95% by HPLC

Physicochemical Information
Cell permeable	Y

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze -20°C. Stock solutions are stable for up to 3 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
5.04043.0001	04055977264258

Documentation

Rho Inhibitor II, Y16 - CAS 429653-73-6 - Calbiochem (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Rho Inhibitor II, Y16 - CAS 429653-73-6 - Calbiochem 試験成績書（CoA）

タイトル	ロット番号
504043	ロット番号を入力

参考資料

参考資料の概要
Shang, X., et al. 2013. Proc. Natl. Acad. Sci. USA. 110, 3155.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	21-June-2013 JSW
Synonyms	LARG Rho-Binding Domain Blocker, (E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenylpyrazolidine-3,5-dione, (4E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenyl-3,5-pyrazolidinedione, p115 Rho-Binding Domain Blocker, PDZ Rho-Binding Domain Blocker
Description	A cell-permeable pyrazolidinedione compound that prevents RhoGEFs LARG, p115, and PDZ from interacting with RhoA, while displaying little potency against DBL-RhoA, LBC-RhoA, intersectin-Cdc42, or TrioN-Cdc42 interaction. Structure and in vitro binding studies using DH-PH domains-containing human LARG catalytic fragment (aa 765-1138) constructs reveals RhoGEF DH-PH domain junction as the high affinity Y16 targeting site (K_d = 65, 472, 2121, and > 5 x 10⁵ nM using wt, K979A, E982A, and N983A LARG, respectively). Shown to prevent serum-induced activation of cellular RhoA, but not Cdc42 or Rac1, in serum-starved NIH-3T3 cultures (215%, 109%, and 87% of basal RhoA-GTP level with 0, 10, or 30 µM Y16, respectively) and effectively inhibit RhoA downstream signaling events, including FAK and MLC phosphorylations and stress fiber formation without apparent cytotoxicity (50 µM up to 24 h) or affecting Cdc42-mediated filapodia and Rac1-mediated lamellipodia formation. Greatly synergizes with Rho GEF-binding domain blocker Rhosin (Cat. No. 555460) in blocking RhoA-LARG binding (8%, 27%, and 72% inhibition, respectively, with 5 µM G04 alone, 5 µM Y16 alone, or in combination) and in inhibiting cellular RhoA activation (80% inhibition of serum-stimulated RhoA-GTP level by 30 µM Y16 alone or by a combination of 5 µM each of G04 and Y16 in serum-starved NIH-3T3 cells).
Form	Orange powder
Intert gas (Yes/No)	Packaged under inert gas
CAS number	429653-73-6
Chemical formula	C₂₄H₂₀N₂O₃
Structure formula
Purity	≥95% by HPLC
Solubility	DMSO (10 mg/ml)
Storage	Protect from light +2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze -20°C. Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Standard Handling
References	Shang, X., et al. 2013. Proc. Natl. Acad. Sci. USA. 110, 3155.

カテゴリー

Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > ATPase/GTPase Inhibitors > GTPase Inhibitors

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