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14-863 Raf-1 RBD Protein, GST, 300 µg

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14-863
300 µg  
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      概要

      Replacement Information
      Description
      Catalogue Number14-863
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRaf-1 RBD Protein, GST, 300 µg
      OverviewGST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity > 50% at full length molecular weight 42 kDa.
      Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
      References
      Product Information
      Presentation300 μg of Raf-1 RBD, GST in 150 μL in PBS, pH 7.4, 0.5mM PMSF, and 20% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationGST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity 50% at full length molecular weight 42 kDa. For use in Affinity Binding Assays
      Key Applications
      • Affinity Binding Assay
      Biological Information
      SourceExpressed in E. coli
      Specific Activity>50% purity<br /> Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
      Entrez Gene Number
      Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
      Gene Symbol
      • RAF1
      • cRaf
      • Raf-1
      • c-Raf
      • RAF
      • CRAF
      • C-RAF
      Purification MethodGlutathione-agarose
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
      COFACTOR: Binds 2 zinc ions per subunit.
      SIZE: 648 amino acids; 73052 Da
      SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight42 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAffinity Precipitation of Activated Ras: Raf-1-RBD, GST can be captured on a glutathione coated plate and used to capture GTP activated Ras from HeLa cell lysate. Used 2 μg of Raf-1-RBD, GST per well to capture 25-100 μg of EGF stimulated HeLa cell lysate. Upstate also offers a Ras GTPase ELISA kit (17-424).
      Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment.
      Packaging Information
      Material Size300 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      14-863 04053252627996

      Documentation

      Raf-1 RBD Protein, GST, 300 µg (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Raf-1 RBD Protein, GST, 300 µg 試験成績書(CoA)

      タイトルロット番号
      Raf-1 RBD, GST - 2444227 2444227
      Raf-1 RBD, GST (recombinant protein expressed in E.coli) - 2318884 2318884
      Raf-1 RBD, GST (recombinant protein expressed in E.coli) - 2193201 2193201
      Raf-1 RBD, GST (recombinant protein expressed in E.coli) - DAM1467234 DAM1467234
      Raf-1 RBD, GST - 1969112 1969112
      Raf-1 RBD, GST - 1992010 1992010
      Raf-1 RBD, GST - 2900316 2900316
      Raf-1 RBD, GST - 3015445 3015445
      Raf-1 RBD, GST - 3315786 3315786
      Raf-1 RBD, GST - 3468019 3468019

      参考資料

      参考資料の概要Pub Med ID
      Comparison of straight chain and cyclic unnatural amino acids embedded in the core of staphylococcal nuclease
      Wynn, R, et al
      Protein Sci, 6:1621-6 (1997)  1997

      9260275 9260275
      Biphasic activation of p21ras by endothelin-1 sequentially activates the ERK cascade and phosphatidylinositol 3-kinase.
      Foschi, M, et al.
      EMBO J., 16: 6439-51 (1997)  1997

      概要を表示する
      9351826 9351826
      Minimal Ras-binding domain of Raf1 can be used as an activation-specific probe for Ras.
      de Rooij, J and Bos, J L
      Oncogene, 14: 623-5 (1997)  1997

      概要を表示する
      9053862 9053862

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      カタログ番号 説明  
      05-1072 Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2 価格&在庫状況を表示

      カテゴリー

      Life Science Research > タンパク質と酵素 > Other Proteins