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ECM509 QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric

The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with fluorescent detection.

QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Key Applications: Activity Assay
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ECM509
1 plate  24 wells
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      概要

      Replacement Information

      主要スペック表

      Key ApplicationsDetection Methods
      ACTFluorescent
      Description
      Catalogue NumberECM509
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

      The CHEMICON® QCM™ 24-well Cell Migration Assay does not require cell labeling, scraping, washing or counting. The 24-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples.

      In the CHEMICON® QCM™ 24-well Migration Assay, invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQUANTâ GR dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3).

      The CHEMICON® QCM™ 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.

      The CHEMICON® QCM™ 24-well Migration Assay utilizes an 8 mm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.



      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

      · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

      · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

      · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

      · QCM™ 96-well Cell Invasion Assay (ECM555)

      · QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)

      · 24-well Insert Cell Migration and Invasion Assay Systems

      · CytoMatrix™ Cell Adhesion strips (ECM protein coated)

      · QuantiMatrix™ ECM protein ELISA kits

      Test Principle

      The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membrane, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.
      Materials Required but Not Delivered1. Precision pipettes: sufficient for aliquoting cells.

      2. Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators.

      Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery.

      3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS.

      4. Chemoattractants (eg. 10% FBS) or pharmacological agents for addition to culture medium, if screening is desired.

      5. Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA.

      Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer.

      6. Sterile PBS or HBSS to wash cells.

      7. Distilled water.

      8. Low speed centrifuge and tubes for cell harvesting.

      9. CO2 incubator appropriate for subject cells.

      10. Hemocytometer or other means of counting cells.

      11. Trypan blue or equivalent viability stain.

      12. Fluorescence plate reader.

      13. Sterile cell culture hood.
      References
      Product Information
      Components
      • Sterile 24-well Cell Migration Plate Assembly: (Part No. 90333) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
      • Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
      • 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
      • CyQUANTâ GR Dye1: (Part No. 90132) One vial - 75 μL
      • Forceps: (Part No. 10203) One each.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationThe QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with fluorescent detection.
      Key Applications
      • Activity Assay
      Application NotesThe CHEMICON® QCM™ 24-well Migration Assay is ideal for the study of chemotaxis cell migration. Each Chemicon Cell Migration Assay Kit contains sufficient reagents for the evaluation of 24 samples. The quantitative nature of this assay is especially useful for screening of pharmacological agents.

      The CHEMICON® QCM™ 24-well Migration Assay is intended for research use only; not for diagnostic applications.
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at 2-8°C for up to their expiration date. Do not freeze.
      Packaging Information
      Material Size1 plate
      Material Package24 wells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ECM509 04053252668852

      Documentation

      QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      参考資料

      参考資料の概要Pub Med ID
      RNAi-mediated silencing of vEGF-C inhibits non-small cell lung cancer progression by simultaneously down-regulating the cXCR4, cCR7, vEGFR-2 and vEGFR-3-dependent axes-induced ERK, p38 and AKT signalling pathways.
      Feng Y, Hu J, Ma J, Feng K, Zhang X, Yang S, Wang W, Zhang J, Zhang Y
      European journal of cancer (Oxford, England : 1990)  2011

      概要を表示する       		21680174 21680174

      カタログ

      タイトル
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
      Cell Migration and Invasion: Choosing the Right Assay
      EpiGRO™, EndoGRO™ and FibroGRO™ Reagents

      データシート

      タイトル
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      カテゴリー

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays