539514 Sigma-AldrichProtein Phosphatase, Lambda, Recombinant, E. coli
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概要
価格&在庫状況
カタログ番号 | 在庫状況 | 包装 | Qty/Pk | 価格 | 数量 | |
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539514-20KUCN |
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樹脂アンプル | 20 ku |
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References | |
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References | Zhuo, S., et al. 1993. J. Biol. Chem. 268, 17754. Gordon, J.A. 1991. Methods Enzymol. 201, 477. Cohen, P.T.W. and Cohen, P. 1989. Biochem. J. 260, 931. |
Product Information | |
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Activity | ≥400,000 units/ml. |
Unit of Definition | One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of <i>p</i>NPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min. |
Form | Liquid |
Formulation | In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5. |
Quality Level | MQ100 |
Biological Information | |
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Purity | ≥95% by SDS-PAGE |
Physicochemical Information | |
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Contaminants | Protease activity: none detected |
Global Trade Item Number | |
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カタログ番号 | GTIN |
539514-20KUCN | 04055977269239 |