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PS1023 PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

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PS1023
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概要

主要スペック表

Species ReactivityHostAntibody Type
HRbMonoclonal Antibody

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PS1023-50ULCN
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      Description
      OverviewRecognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
      Catalogue NumberPS1023
      Brand Family Calbiochem®
      Application Data
      Detection of human Smad3, phosphorylated at Ser423/425, by immunoblotting. Samples: whole cell lysate from HT-1080 cells, left untreated or treated with TGF-β1, TGFR inhibitor (SB-431542), or BMP-2, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) (Cat. No. PS1023) (1:1000) (top panel) and Anti-Smad3 (total) (bottom panel). Detection: chemiluminescence.
      References
      ReferencesAttisano, L., et al. 2002. Science 296, 1646.
      Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
      Wrana, J. 2000. Science 23, 1.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (1:50)
      Application CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.
      CloneC25A9
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Global Trade Item Number
      カタログ番号 GTIN
      PS1023-50ULCN 04055977227116