数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

525295 Sigma-AldrichPhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20)

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
A Broad Range Of Species	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
525295-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Catalogue Number	525295
Brand Family	Calbiochem®

References
References	Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.

Product Information
Form	Liquid
Formulation	In 10 mM sodium phosphate, pH 7.4.
Positive control	Any cell lysate or tissue extract
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.
Key Applications	Enzyme-Linked Immunosorbent Assay Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	ELISA (see comments) Immunoblotting (1:500-1:1000, see application references) Frozen Sections (see application references) Immunocytochemistry (see application references) Immunoprecipitation (see comments)
Application Comments	Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	KLH conjugated to phosphotyrosine, glycine, and alanine
Clone	PY20
Host	Mouse
Isotype	IgG_2b
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
525295-100UGCN	04055977270686

Documentation

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) 試験成績書（CoA）

タイトル	ロット番号
525295	ロット番号を入力

参考資料

参考資料の概要
Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	03-December-2007 JSW
Application	ELISA (see comments) Immunoblotting (1:500-1:1000, see application references) Frozen Sections (see application references) Immunocytochemistry (see application references) Immunoprecipitation (see comments)
Description	Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Background	Cellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
Host	Mouse
Immunogen	KLH conjugated to phosphotyrosine, glycine, and alanine
Clone	PY20
Isotype	IgG_2b
Species	a broad range of species
Positive control	Any cell lysate or tissue extract
Form	Liquid
Formulation	In 10 mM sodium phosphate, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Application references	Immunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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