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407707 PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

This PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr1162/1163).

PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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407707
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概要

主要スペック表

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

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407707-10TCN
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      		 樹脂ｱﾝﾌﾟﾙ 10 t
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      Description
      OverviewRecognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
      Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
      Catalogue Number407707
      Brand Family Calbiochem®
      Application Data
      Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
      References
      ReferencesBevan, P. 2001. J. Cell Sci. 114, 1429.
      Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
      Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
      Schmid, E., at al. 1998. FASEB J. 12, 863.
      Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
      Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
      White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
      Ebina, Y., et al. 1985. Cell 40, 747.
      Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
      Grunberger, G., et al. 1984. Science 223, 932.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Application CommentsThe immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      カタログ番号 GTIN
      407707-10TCN 04055977211474