Millipore Sigma Vibrant Logo

69959 Perfect Protein™ Western Markers

View This Product on Sigma-Aldrich
69959
価格&在庫状況を照会

概要

価格&在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
69959-3CN
現在国内在庫なし
現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      樹脂アンプル 25 lane
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示 
      値引
      ()
       
      弊社照会
      Description
      Overview

      The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

      For ease of convenience, please consider the Perfect Protein AP Western Blot Kit (Cat. No. 69965), or the Perfect Protein HRP Western Blot Kit (Cat. No. 69078). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.


      A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
      B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.

      Catalogue Number69959
      Brand Family Novagen®
      Features and benefits• Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection.
      • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
      • Can be used with colorimetric and chemiluminescent AP or HRP substrates.
      • Recombinant, unmodified markers give sharp, accurately sized bands.
      • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
      • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
      Product Information
      FormLiquid
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Toxic
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      カタログ番号 GTIN
      69959-3CN 04055977274059