Millipore Sigma Vibrant Logo

539481 PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit

View This Product on Sigma-Aldrich
539481
価格＆在庫状況を照会

概要

Replacement Information

価格＆在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
539481-100KUCN
在庫状況検索中…
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      		 樹脂ｱﾝﾌﾟﾙ 100 ku
      価格を検索中…
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示　
      値引
      ()
       
      —
      弊社照会
      		 —
      Description
      OverviewRecombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
      Catalogue Number539481
      Brand Family Calbiochem®
      SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      References
      ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
      Product Information
      Activity≥2.5 x 10⁶ U/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; <a href ="/Products/ProductDisplay.asp→catNO=05-23-4900">Cat. No. 05-23-4900</a>) per min at 30°C, pH 7.5.
      EC number2.7.1.37
      FormLiquid
      FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsPhosphatases, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      539481-100KUCN 04055977269178

      Documentation

      PKA, Catalytic Subunit, Mouse, Recombinant, E. coli (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      PKA, Catalytic Subunit, Mouse, Recombinant, E. coli 試験成績書（CoA）

      タイトルロット番号
      539481

      参考資料

      参考資料の概要
      Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.

      引用

      Title
    • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).
      		•
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-January-2008 JSW
      SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      DescriptionRecombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP3-induced Ca2+ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.
      FormLiquid
      FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
      Recommended reaction conditions50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 200 µM ATP, and γ-labeled-32P-ATP to a final specific activity of 200 μCi/μmol. Incubate at 30°C.
      EC number2.7.1.37
      ContaminantsPhosphatases, proteases: none detected
      Activity≥2.5 x 10⁶ U/ml
      Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
      Citation
    • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).
      		•