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14-901 PIP4K2α Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant human PIP4K2α, full length. For use in Kinase Assays.

PIP4K2α Protein, active, 10 µg: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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14-901
10 µg  Also available in 250μg size as catalog # 14-901M - Call for pricing and availability.
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      概要

      Replacement Information
      Description
      Catalogue Number14-901
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPIP4K2α Protein, active, 10 µg
      OverviewN-terminal 6His-tagged, recombinant human PIP4K2α, full length.
      References
      Product Information
      Presentation10µg of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant human PIP4K2α, full length. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
      Gene Symbol
      • PIP4K2A
      • PIP5K2
      • PIP5K2A
      Protein TargetPIP4K2α
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyPIPK
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
      CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
      SUBUNIT STRUCTURE: Homodimer
      TISSUE SPECIFICITY: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
      SIMILARITY: Contains 1 PIPK domain.
      Molecular Weight51kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in the PIProfiler.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size as catalog # 14-901M - Call for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      14-901 04053252402692

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      カテゴリー

      Life Science Research > タンパク質と酵素 > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases