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14-783 PI3 Kinase (p110α(E545K)/p85α), 10 µg

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E545K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.

PI3 Kinase (p110α(E545K)/p85α), 10 µg: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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14-783
10 µg  Also available in 250 μg size --call for pricing and availability and reference catalog number 14-783M when ordering the 250 μg size.
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      概要

      Replacement Information
      Description
      Catalogue Number14-783
      Trade Name
      • Upstate
      DescriptionPI3 Kinase (p110α(E545K)/p85α), 10 µg
      OverviewComplex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E545K and untagged, recombinant, full-length human p85α.
      Background InformationThe E545K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationComplex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E545K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceCoexpressed by baculovirus in Sf21 insect cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • PI3K
      • MGC142161
      • p110-alpha
      • MGC142163
      Protein TargetPI3Kα/p85α
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Molecular Weightp110α (E545K) MW = 125.3kDa, p85α MW = 83.7kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 μg size --call for pricing and availability and reference catalog number 14-783M when ordering the 250 μg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      14-783 04053252270932

      Documentation

      PI3 Kinase (p110α(E545K)/p85α), 10 µg (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      PI3 Kinase (p110α(E545K)/p85α), 10 µg 試験成績書（CoA）

      タイトルロット番号
      PI 3-Kinase (p110#945;(E545K)/p85#945;) - 1654888-C 1654888-C
      PI 3-Kinase (p110α(E545K)/p85α) - 1654888-D 1654888-D

      技術情報

      タイトル
      Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

      データシート

      タイトル
      PI 3-Kinase HTRF Assay

      関連製品＆アプリケーション

      Alternative Packsize

      カタログ番号 説明  
      14-783M PI3 Kinase (p110α(E545K)/p85α), 250 µg 価格＆在庫状況を表示

      Accessories

      カタログ番号 説明  
      14-558 PI3 Kinase (p120γ), 20 µg 価格＆在庫状況を表示
      14-602 PI3 Kinase (p110α/p85α) Protein, active, 20 µg 価格＆在庫状況を表示
      14-603 PI3 Kinase (p110β/p85α) Protein, active, 20 µg 価格＆在庫状況を表示
      14-604 PI3 Kinase (p110δ/p85α) Protein, active, 10 µg 価格＆在庫状況を表示
      14-781 PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg 価格＆在庫状況を表示
      14-782 PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg 価格＆在庫状況を表示
      14-785 PI3 Kinase (p110α/p85α) murine, 10 µg 価格＆在庫状況を表示
      14-786 PI3 Kinase (p110α/p65α) Murine, 10 µg 価格＆在庫状況を表示
      14-787 PI3 Kinase (p110α (H1047R)/p85α) Murine, 10 µg 価格＆在庫状況を表示
      14-788 PI3 Kinase (p110β/p85β) Murine, 10 µg 価格＆在庫状況を表示
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      カテゴリー

      Life Science Research > タンパク質と酵素 > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases