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69831 Mouse Ig-Primer Set

Mouse Ig-Primer Set: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
69831
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69831-3CN
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      Description
      OverviewThe Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.

      In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.

      Please call Technical Service if you need more information regarding individual Ig-Primer Sets.
      Catalogue Number69831
      Brand Family Novagen®
      References
      ReferencesLarrick, J.W., et al. 1989. Biochem. Biophys. Res.
      Comm.       160, 1250. Jones, S.T. and Bendig, M.M. 1991.
      Biotechnology 9, 88.
      Product Information
      •500 pmolMu IgM VH 3ʹ-1 Primer
      •500 pmolMu IgG VH 3ʹ-2 Primer
      •500 pmolMu Ig VH 5ʹ-A Primer
      •500 pmolMu Ig VH 5ʹ-B Primer
      •100 pmolMu Ig VH 5ʹ-C Primer
      •100 pmolMu Ig VH 5ʹ-D Primer
      •100 pmolMu Ig VH 5ʹ-E Primer
      •100 pmolMu Ig VH 5ʹ-F Primer
      •500 pmolMu Igκ VL 3ʹ-1 Primer
      •500 pmolMu Igκ VL 5ʹ-A Primer
      •500 pmolMu Igκ VL 5ʹ-B Primer
      •100 pmolMu Igκ VL 5ʹ-C Primer
      •100 pmolMu Igκ VL 5ʹ-D Primer
      •100 pmolMu Igκ VL 5ʹ-E Primer
      •100 pmolMu Igκ VL 5ʹ-F Primer
      •100 pmolMu Igκ VL 5ʹ-G Primer
      •500 pmolMu Igλ VL 3ʹ-1 Primer
      •500 pmolMu IgMλ VL 5ʹ-A Primer
      •10 µlMouse Positive Control PCR Template
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      69831-3CN 04055977274011

      Documentation

      Mouse Ig-Primer Set 試験成績書（CoA）

      タイトルロット番号
      69831

      参考資料

      参考資料の概要
      Larrick, J.W., et al. 1989. Biochem. Biophys. Res.
      Comm.       160, 1250. Jones, S.T. and Bendig, M.M. 1991.
      Biotechnology 9, 88.

      引用

      Title
    • Emi Hifumi, et al. (2008) Catalytic features and eradication ability of antibody light-chain UA15-L against Helicobacter pylori. Journal of Biological Chemistry 283, 899-907.
    • Anna S. Gardberg, et al. (2007) Molecular basis for passive immunotherapy of Alzheimer's disease. Procedings of the National Academy of Science 104, 15659-15664.
    • Kazuyo Toyoda, et al. (2007) Protein-bound 4-hydroxy-2-nonenal: an endogenous triggering antigen of anti-DNA response. Journal of Biological Chemistry 282, 25769-25778.
    • Manisha Goel, et al. (2004) Plasticity within the antigen-combining site may manifest as molecular mimicry in the humoral immune response. Journal of Immunology 173, 7358-7367.
    • Eric A. Grovender, et al. (2004) Single-chain antibody fragment-based Adsorbent for the extracorporeal removal of β2-microglobulin. Kidney International 65, 310-322.
    • Michael G. Rosenblum, et al. (2003) Design, expression, purification, and characterization, in vitro and in vivo, of an antimelanoma single-chain Fv antibody fused to the toxin gelonin. Cancer Research 63, 3995-4002.
    • Lingxun Duan, et al. (1994) Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody. Procedings of the National Academy of Science 91, 5075-5079.
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