Millipore Sigma Vibrant Logo

APT242 MitoLight® Mitochondrial Apoptosis Detection Kit

The MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker.

MitoLight® Mitochondrial Apoptosis Detection Kit: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Key Applications: Flow Cytometry
View This Product on Sigma-Aldrich
APT242
100 assays  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

       

      お問合せください

      概要

      Replacement Information

      主要スペック表

      Key ApplicationsDetection Methods
      FCFluorescent
      Description
      Catalogue NumberAPT242
      Brand Family Chemicon®
      Trade Name
      • MitoLight
      • Chemicon
      DescriptionMitoLight® Mitochondrial Apoptosis Detection Kit
      OverviewDisruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.

      MitoLight™ partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratioing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (λem = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (λem = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585).
      Materials Required but Not Delivered· Microcentrifuge and 1.5 mL Microcentrifuge tubes

      · 37°C CO2 incubator

      · Flow Cytometer

      · Glass slides with glass coverslips
      References
      Product Information
      Components
      • MitoLight™ (Part No. 71616): 100 μL in DMSO.
      • 10X Incubation Buffer (Part No. 71614): 20 mL.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationThe MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Mammals
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at -20°C up to their expiration date. After thawing, unused kit components can be refrozen. For best results, aliquot the MitoLight™ reagent into separate vials to avoid repeated freeze/thaw cycles.

      PROTECT REAGENTS FROM LIGHT.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      APT242 08436037125430

      関連製品＆アプリケーション

      製品ファミリー

      Apoptosis & Cell Death Assays

      We offer a significant portfolio of well-published, quantitative and optimized whole-cell, and cell-based apoptosis assays for the study of Apoptosis and Cell Death.
      詳細はこちら >>

      Cell Based Assays

      Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
      詳細はこちら >>

      関連製品: Brand Facete

      MitoLight®

      カテゴリー

      Life Science Research > Cell Analysis > Cell-based Assays > Apoptosis / Cell Death Assays