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FCMAB191F Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271

This Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.

Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology UniProt Number: P26842
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FCMAB191F
100 tests  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB191F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-CD27-FITC Antibody, clone M-T271
      Alternate Names
      • CD27 antigen
      • CD27L receptor
      • T-cell activation antigen CD27
      • T14
      • Tumor necrosis factor receptor superfamily member 7
      Background InformationCD27, a member of the nerve growth factor receptor (NGFR) superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kDa homodimer (Bigler, 1988; Sugita, 1992; Morimoto, 1995). CD27 is expressed on mature thymocytes and on the majority of human peripheral blood T cells, on both CD4+ and CD8+ subsets. CD27 is also expressed on activated B cells and a fraction of resting natural killer (NK) cells (Maurer, 1990; Sugita, 1992). Among CD4+ T cells, CD27 is preferentially expressed on unprimed CD4+CD45RA+CD45R0- T cells, while primed CD4+CD45RA-CD45R0+ T cells express low levels of CD27 (Sugita, 1992). During activation, the expression of CD27 is increased on B cells and unprimed T cells, while primed T cells have a relatively low capacity for upregulation of CD27 (Morimoto, 1995; Hintzen, 1994). The activation is also accompanied by the appearance of a 32 kDa soluble form of CD27 (sCD27) found in supernatants of activated lymphocytes (Hintzen, 1994).
      CD27 is believed to play a role in T cell activation, since the expression is upregulated during T cell activation (Bigler, 1988; Sugita, 1992; Morimoto, 1995; Maurer, 1990; Hintzen, 1994; Lens, 1995).
      References
      Product Information
      FormatFITC
      Control
      • PBMCs
      PresentationPurified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
      Quality LevelMQ100
      Applications
      ApplicationThis Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenLeukemic peripheral blood T cells from a patient with chronic T cell leukemia (T-CLL)
      CloneM-T271
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibody recognizes Human CD27 antigen.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight120 kDa (Bigler, 1988; Sugita, 1992; Morimoto, 1995)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using human PBMCs
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      FCMAB191F 04053252289163

      Documentation

      Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271 試験成績書（CoA）

      タイトルロット番号
      Milli-Mark Anti-CD27-FITC, clone M-T271 - 2007336 2007336
      Milli-Mark Anti-CD27-FITC, clone M-T271 - NRG1761334 NRG1761334
      Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 2845085 2845085
      Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 3503850 3503850
      Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 4113040 4113040
      Milli-Mark™ Anti-CD27-FITC, clone M-T271 -2859521 2859521
      Milli-Mark™ Anti-CD27-FITC, clone M-T271 - 2464626 2464626

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      製品ファミリー

      Flow Cytometry Kits & Antibodies

      Merck's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
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      Life Science Research > Antibodies and Assays > Primary Antibodies