Microcon® Centrifugal Filters for Protein and DNA Concentration
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関連製品＆アプリケーション
関連製品: Application Facete
| Protein Purification |
| DNA Purification |
| Dialysis and Filtration |
関連製品: Brand Facete
| Ultracel® |
カテゴリー
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
Microcon® Centrifugal Filters
Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:
- Typical recoveries of >95%, even for dilute solutions
- Reverse spin to maximize recovery, even in the smallest samples
- Convenient storage of filtrate or concentrated sample in standard microfuge tube
- Concentration factors up to 100X
Microcon® DNA Fast Flow Filter
Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.
Features:
- High recovery for small volumes with reverse spin (concentration factor <20X)
- Low-binding Ultracel® membrane
- Fast processing
Sample applications:
- Recovery of genomic DNA for forensic applications
- Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
- Removal of primers from amplified DNA
- Easy filtrate recovery, exploiting the flat membrane design
| Microcon® Device | |||
| Application | 10K | 30K | DNA Fast Flow |
| Peptide and growth factor concentration | ♦ | ||
| Protein concentration and desalting of columns eluates | ♦ | ♦ | |
| Protein concentration before electrophoresis or other assays | ♦ | ♦ | |
| Protein removal prior to HPLC | ♦ | ♦ | |
| Purification of macromolecular components found in tissue culture extracts and cell lysates | ♦ | ♦ | |
| Concentration of biological samples (antigens, antibodies, enzymes) | ♦ | ||
| Concentration of gDNA with or without SDS buffer | ♦ | ♦ | |
| Concentration and desalting of nucleic acids (single-or double-stranded) | ♦ | ♦ | ♦ |
| Removal of labeled nucleotides | ♦ | ♦ | ♦ |
| Removal of labeled amino acids | ♦ | ♦ | ♦ |
| Removal of primers from amplified DNA | ♦ | ♦ | |
| Removal of linkers prior to cloning | ♦ | ♦ | |
Centrifugal Filter Units
Centrifugal Filter Units
| Volumes | |
| Maximum initial sample volume, µL | 0.5 ml |
| Typical final concentration volume, µL | 5 -50 μL |
| OPTIMAL and maximum relative centrifugal force – Fixed angle rotor | Microcon 10K devices - 14,000 x g Microcon 30K devices - 14,000 x g Microcon DNA Fast Flow - 500 x g 1,000 x g for recovery spin |
| Active membrane area, cm2 | 0.32 cm2 |
| Hold up volume, µL | ≤10 μL |
| Materials | |
| Filter device | Polycarbonate |
| Membrane | Ultracel low-binding regenerated cellulose |
| Membrane support base | Acetal |
| Collection tube | Polypropylene |
| O-Ring | Medical-grade silicone rubber |
| Dimensions | |
| Diameter, mm (in.) | 12.3 mm, (0.5 in.) |
| Length, mm (in.) (filter device and tube in concentration mode) | 45.0 mm (1.8 in.) |
| Length, mm (in.) (filter device and tube in recovery mode) | 48.2 mm (1.9 in.) |