Millipore Sigma Vibrant Logo

475935 MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli

MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: MMP-14 Catalytic Domain

View This Product on Sigma-Aldrich
475935
価格＆在庫状況を照会

概要

Replacement Information

価格＆在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
475935-10UGCN
在庫状況検索中…
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      		 樹脂ｱﾝﾌﾟﾙ 10 μg
      価格を検索中…
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示　
      値引
      ()
       
      —
      弊社照会
      		 —
      Description
      OverviewRecombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). Degrades fibrillar collagen Types I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13 resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
      Note: 1 mU = 1 milliunit.
      Catalogue Number475935
      Brand Family Calbiochem®
      SynonymsMMP-14 Catalytic Domain
      References
      ReferencesAmano, T., et al. 2005. Cell Res. 15, 150.
      Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
      Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
      Sato, H., et al. 1994. Nature 370, 61.
      Knight, C.G., et al. 1992. REBS Lett. 296, 263.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (<a href ="/Products/ProductDisplay.asp→catNO=03-32-5032">Cat. No. 03-32-5032</a>) per min at 37°C, pH 7.5.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥140 mU/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      475935-10UGCN 04055977184235

      Documentation

      MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli 試験成績書（CoA）

      タイトルロット番号
      475935

      参考資料

      参考資料の概要
      Amano, T., et al. 2005. Cell Res. 15, 150.
      Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
      Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
      Sato, H., et al. 1994. Nature 370, 61.
      Knight, C.G., et al. 1992. REBS Lett. 296, 263.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-September-2007 RFH
      SynonymsMMP-14 Catalytic Domain
      DescriptionRecombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). The catalytic domain is comprised of amino acid residues Tyr89 to Gly265 of mature human MT1-MMP. Hydrolyzes fibrillar collagens I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13, resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Activity Assay
      Reagent Preparation and Required Equipment Peptide Hydrolysis Buffer: 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, 0.025 % Brij® 35 detergent, pH 7.5. Store at 4°C; stable for several weeks. Peptide Substrate Stock: 100 µM Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 05-32-5032) in 20% DMSO. Store at -20°C. Peptide Standard: 10 µM (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-NH2 (Mca-Pro-Leu) (Cat. No. 05-32-5033) in 20% DMSO. Store at -20°C. Fluorimeter: Excitation wavelength: ~328 nm; emission wavelength: ~393 nm
      Protocol Note: This protocol is provided only as a guide; assay conditions should be optimized for individual experiments.
      The protocol is based on the following reference: Knight, C.G., et al. 1992. FEBS Lett. 296, 263.
      1. Calibrate the fluorimeter using the Peptide Standard (Mca-Pro-Leu) at a concentration that corresponds to 2–10% hydrolysis of the Peptide Substrate. 2. Kinetic Dilute the Peptide Substrate Stock to 8 µM in Peptide Hydrolysis Buffer and equilibrate to 37°C. 3. Add 2–4 µl MT1-MMP and record the increase in fluorescence at desired intervals from 2–12 min. 4. Calculate activity in units per ml enzyme using the following equation: Activity (U/ml) = cMca-Pro-Leu/FMca-Pro-Leu x ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg/venzyme x Vtotal cMca-Pro-Leu: Concentration of Mca-Pro-Leu used for calibration of the fluorimeter (µmol/ml) FMca-Pro-Leu: Fluorescence of Mca-Pro-Leu at the concentration cMca-Pro-Leu used for fluorimeter calibration ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg: Change in fluorescence during peptide hydrolysis per min V: Volume of peptide hydrolysis reaction (2.5 ml) v: Volume of added enzyme (2–4 µl)
      Purity≥90% by SDS-PAGE
      Specific activity≥140 mU/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesAmano, T., et al. 2005. Cell Res. 15, 150.
      Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
      Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
      Sato, H., et al. 1994. Nature 370, 61.
      Knight, C.G., et al. 1992. REBS Lett. 296, 263.