数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

444232 Sigma-AldrichMMP-9, Dimer, Human Neutrophil

MMP-9, Dimer, Human Neutrophil: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase

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概要

Replacement Information

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
444232-5UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	5 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.
Overview	M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.
Catalogue Number	444232
Brand Family	Calbiochem®
Synonyms	Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase

References
References	Olson, M.W. et al. 2000. J. Biol. Chem. 275, 2661. Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Product Information
Unit of Definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
EC number	3.4.24.35
Form	Liquid
Formulation	In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.
Quality Level	MQ100

Applications

Biological Information
Purity	≥90% by SDS-PAGE
Source	Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity	≥1000 mU/mg protein

Physicochemical Information
Contaminants	No other MMP activity detectable

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
444232-5UGCN	04055977186390

Documentation

MMP-9, Dimer, Human Neutrophil (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

MMP-9, Dimer, Human Neutrophil 試験成績書（CoA）

タイトル	ロット番号
444232	ロット番号を入力

参考資料

参考資料の概要
Olson, M.W. et al. 2000. J. Biol. Chem. 275, 2661. Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	18-July-2007 RFH
Synonyms	Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
Description	Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.
Form	Liquid
Formulation	In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.
Recommended reaction conditions	Organomercurial Activation Protocol This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. The following protocol is from Stricklin, et al., which describes the use of p-aminophenylmercuric acetate (APMA) to activate pro-MMP. This protocol is also adaptable to other types of organomercurals, such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), or mersalyl. 1. Prepare a 10-50 mM stock solution of APMA (or other organomercurial compound) in 0.1 M NaOH just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl (see Marcy, A.I., et al.). 2. To initiate the activation mix the proenzyme solution with the APMA solution at a 10:1 volume ratio (MMP:APMA). If a higher concentration of APMA is desired, increase the concentration of the stock solution. Do not exceed the 10:1 ratio, as this could result in significant changes in pH. 3. Incubate the mixture at 37°C for 2-3 h. It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with a fixed concentration of pro-MMP and organomercurial would be incubated as described above. Remove aliquots of the sample at various time points during the incubation. Stop the reaction by the addition of SDS-PAGE sample buffer (e.g., 10 µl 2X sample buffer to 10 µl aliquot) and heat the samples to 95°C. The progress of activation can be monitored qualitatively by analyzing the aliquots on a 12% SDS-PAGE gel. 4. The activated MMP can be used without removing the APMA from the mixture. Please refer to Marcy, A.I., et al. for removal of organomercurials by gel filtration.
Source	Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
EC number	3.4.24.35
Purity	≥90% by SDS-PAGE
Contaminants	No other MMP activity detectable
Specific activity	≥1000 mU/mg protein
Unit definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).
Toxicity	Standard Handling
References	Olson, M.W. et al. 2000. J. Biol. Chem. 275, 2661. Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Alternative Products

カタログ番号

説明

444231

MMP-9, Monomer, Human Neutrophil

価格＆在庫状況を表示

カテゴリー

Life Science Research > タンパク質と酵素 > Other Enzymes

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