Millipore Sigma Vibrant Logo

71259 Insect GeneJuice® Transfection Reagent

View This Product on Sigma-Aldrich
71259
価格&在庫状況を照会

概要

Replacement Information

価格&在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
71259-3CN
在庫状況検索中…
現在国内在庫なし
現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      樹脂アンプル 0.3 ml
      価格を検索中…
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示 
      値引
      ()
       
      弊社照会
      71259-4CN
      在庫状況検索中…
      現在国内在庫なし
現在国内在庫なし
      現在国内在庫有り 
      販売中止
      在庫僅少
      現在国内在庫あり
        Remaining : Will advise
          Remaining : Will advise
          注文対象外
          お問合せください
          Contact Customer Service

          樹脂アンプル 1 ml
          価格を検索中…
          価格が見つかりません
          Minimum Quantity needs to be mulitiple of
          Maximum Quantity is
          弊社照会 詳細を表示 
          値引
          ()
           
          弊社照会
          71259-5CN
          在庫状況検索中…
          現在国内在庫なし
現在国内在庫なし
          現在国内在庫有り 
          販売中止
          在庫僅少
          現在国内在庫あり
            Remaining : Will advise
              Remaining : Will advise
              注文対象外
              お問合せください
              Contact Customer Service

              樹脂アンプル 10 ml
              価格を検索中…
              価格が見つかりません
              Minimum Quantity needs to be mulitiple of
              Maximum Quantity is
              弊社照会 詳細を表示 
              値引
              ()
               
              弊社照会
              Description
              Overview Insect GeneJuice® Transfection Reagent is a proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for cotransfection of transfer plasmids with linearized virus DNA for the production of recombinant baculoviruses. Insect GeneJuice is ideal for large-scale protein expression in suspension-culture transfections of Sf9 insect cells when using pIEx™ and pBiEx™ vectors, which contain the immediate early baculovirus promoter, IE1.

              Insect GeneJuice Transfection Reagent is provided as a 2 mg/ml suspension in 20 mM MES, 150 mM NaCl, pH 6.2 buffer. One ml is sufficient for 12 or 125 transfections in 10-ml suspension culture flasks or 35-mm plates, respectively.

              Request a Free Sample



              Catalogue Number71259
              Brand Family Novagen®
              References
              Product Information
              DeclarationGeneJuice is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
              Quality LevelMQ100
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              カタログ番号 GTIN
              71259-3CN 07790788053123
              71259-4CN 04055977270860
              71259-5CN 04055977270877

              Documentation

              Insect GeneJuice® Transfection Reagent (M)SDS

              タイトル

              英語版製品安全データシート((M)SDS) 

              Insect GeneJuice® Transfection Reagent 試験成績書(CoA)

              タイトルロット番号
              71259

              カタログ

              タイトル
              Bulk Product Guide
              Insect Directル Protein Expression & Purification System
              The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
              Transfection: A Science and an Art

              引用

              Title
            • Jessica K. Bell, et al. (2006) The dsRNA binding site of human Toll-like receptor 3. Procedings of the National Academy of Science 103, 8792-8797.
            • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
            • Hsien-Sheng Yin, et al. (2005) Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein. Proceedings of the National Academy of Sciences (USA) 102, 9288-9293.
            • Gillette, W, et al. (2019) Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System. Methods Mol Biol 2009, 259-277
            • ;
            • Al-Sulaiman, AM, et al. (2017) Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell. Saudi J Biol Sci 24, 1497-1504
            • ;
            • Zimmer, CT, et al. (2018) Neofunctionalization of Duplicated P450 Genes Drives the Evolution of Insecticide Resistance in the Brown Planthopper. Curr Biol 28, 268-274.e5
            • ;
            • Matsuo, E, et al. (2018) Interaction between a Unique Minor Protein and a Major Capsid Protein of Bluetongue Virus Controls Virus Infectivity. J Virol 92,
            • ;
            • Akhmetova, K, et al. (2018) Phosphorylation of Pnut in the Early Stages of Drosophila Embryo Development Affects Association of the Septin Complex with the Membrane and Is Important for Viability. G3 (Bethesda) 8, 27-38
            • ;
            • Zhou, X, et al. (2017) Vaccination with Recombinant Baculovirus Expressing Ranavirus Major Capsid Protein Induces Protective Immunity in Chinese Giant Salamander, Andrias davidianus. Viruses 9,
            • ;
            • Gati, C, et al. (2017) Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser. Proc Natl Acad Sci U S A 114, 2247-2252
            • ;
            • Dhungel, B, et al. (2016) Distal-less induces elemental color patterns in Junonia butterfly wings. Zoological Lett 2, 4
            • ;
            • Hussmann, F, et al. (2016) Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism. Sci Rep 6, 20653
            • ;
            • Alshaikhahmed, K, et al. (2016) Generation of virus-like particles for emerging epizootic haemorrhagic disease virus: Towards the development of safe vaccine candidates. Vaccine 34, 1103-8
            • ;
            • Wangkanont, K, et al. (2016) Structures of Xenopus Embryonic Epidermal Lectin Reveal a Conserved Mechanism of Microbial Glycan Recognition. J Biol Chem 291, 5596-610
            • ;
            • Sacre, S, et al. (2016) Oligodeoxynucleotide inhibition of Toll-like receptors 3, 7, 8, and 9 suppresses cytokine production in a human rheumatoid arthritis model. Eur J Immunol 46, 772-81
            • ;
            • Hirota, Y, et al. (2015) Functional characterization of the vitamin K2 biosynthetic enzyme UBIAD1. PLoS One 10, e0125737
            • ;
            • Lozano, J, et al. (2015) MiR-2 family regulates insect metamorphosis by controlling the juvenile hormone signaling pathway. Proc Natl Acad Sci U S A 112, 3740-5
            • ;
            • Pan, C, et al. (2015) Expression of TAT recombinant Oct4, Sox2, Lin28, and Nanog proteins from baculovirus-infected Sf9 insect cells. Gene 556, 245-8
            • ;
            • Cheng, G, et al. (2014) Structure-function analysis of porcine cytochrome P450 3A29 in the hydroxylation of T-2 toxin as revealed by docking and mutagenesis studies. PLoS One 9, e106769
            • ;
            • Rasool, A, et al. (2014) An independent occurrence of the chimeric P450 enzyme CYP337B3 of Helicoverpa armigera confers cypermethrin resistance in Pakistan. Insect Biochem Mol Biol 53, 54-65
            • ;
            • Kasai, S, et al. (2014) Mechanisms of pyrethroid resistance in the dengue mosquito vector, Aedes aegypti: target site insensitivity, penetration, and metabolism. PLoS Negl Trop Dis 8, e2948
            • ;
            • Cheng, G, et al. (2014) Development of an enzyme-linked-receptor assay based on Syrian hamster Β-adrenergic receptor for detection of &Beta:-agonists. Anal Biochem 459, 18-23
            • ;
            • Sguazza, GH, et al. Expression of the hemagglutinin HA1 subunit of the equine influenza virus using a baculovirus expression system. Rev Argent Microbiol 45, 222-8
            • ;
            • Fabrick, JA, et al. (2014) Molecular and functional characterization of multiple aquaporin water channel proteins from the western tarnished plant bug, Lygus hesperus. Insect Biochem Mol Biol 45, 125-40
            • ;
            • Oloomi, M, et al. (2013) Construction of a Baculovirus vector containing A subunit of Shiga toxin for protein delivery. Iran J Microbiol 5, 350-5
            • ;
            • Yi, X, et al. (2014) Identification of a novel interacting partner of the chemosensory protein 1 from Plutella xylostella L. Int J Biol Macromol 63, 233-9
            • ;
            • Hirota, Y, et al. (2013) Menadione (vitamin K3) is a catabolic product of oral phylloquinone (vitamin K1) in the intestine and a circulating precursor of tissue menaquinone-4 (vitamin K2) in rats. J Biol Chem 288, 33071-80
            • ;
            • Chen, C, et al. (2013) Mechanism of porcine liver xanthine oxidoreductase mediated N-oxide reduction of cyadox as revealed by docking and mutagenesis studies. PLoS One 8, e73912
            • ;
            • Vidmar, T, et al. (2013) Biochemical and preliminary X-ray characterization of the tumor-associated calcium signal transducer 2 (Trop2) ectodomain. Protein Expr Purif 91, 69-76
            • ;
            • Pauchet, Y, et al. (2013) The genome of the mustard leaf beetle encodes two active xylanases originally acquired from bacteria through horizontal gene transfer. Proc Biol Sci 280, 20131021
            • ;
            • Seelhorst, K, et al. (2013) N-glycosylations of human α1,3-fucosyltransferase IX are required for full enzyme activity. Glycobiology 23, 559-67
            • ;
            • Jouβen, N, et al. (2012) Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. Proc Natl Acad Sci U S A 109, 15206-11
            • ;
            • Jiang, X, et al. (2012) Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor. Proc Natl Acad Sci U S A 109, 12491-6
            • ;
            • Taniuchi, K, et al. (2012) BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1. Neoplasia 14, 440-50
            • ;
            • Radner, S, et al. (2012) Transient transfection coupled to baculovirus infection for rapid protein expression screening in insect cells. J Struct Biol 179, 46-55
            • ;
            • Liu, H, et al. (2012) The mechanism of shared but distinct CSF-1R signaling by the non-homologous cytokines IL-34 and CSF-1. Biochim Biophys Acta 1824, 938-45
            • ;
            • Taniuchi, K, et al. (2012) BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7. PLoS One 7, e35674
            • ;
            • Navarro-Cerrillo, G, et al. (2012) Functional interactions between members of the REPAT family of insect pathogen-induced proteins. Insect Mol Biol 21, 335-42
            • ;
            • Shimizu, Y, et al. (2012) Molecular cloning, functional expression, and characterization of isolectin genes of hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata. Biosci Biotechnol Biochem 76, 276-82
            • ;
            • Lara-Gonzalez, P, et al. (2011) BubR1 blocks substrate recruitment to the APC/C in a KEN-box-dependent manner. J Cell Sci 124, 4332-45
            • ;
            • Ridder, MC, et al. (2011) Megalencephalic leucoencephalopathy with cysts: defect in chloride currents and cell volume regulation. Brain 134, 3342-54
            • ;
            • Unger, T, et al. (2012) Recombinant protein expression in the baculovirus-infected insect cell system. Methods Mol Biol 800, 187-99
            • ;
            • Ashikawa, Y, et al. (2011) GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs. Protein Sci 20, 1720-34
            • ;
            • Pfalz, M, et al. (2011) Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification. Plant Cell 23, 716-29
            • ;
            • Mathew, LG, et al. (2011) Identification and characterization of functional aquaporin water channel protein from alimentary tract of whitefly, Bemisia tabaci. Insect Biochem Mol Biol 41, 178-90
            • ;
            • Liu, H, et al. (2011) Homophilic adhesion mechanism of neurofascin, a member of the L1 family of neural cell adhesion molecules. J Biol Chem 286, 797-805
            • ;
            • Nakagawa, K, et al. (2010) Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme. Nature 468, 117-21
            • ;
            • Raymond, WW, et al. (2010) How immune peptidases change specificity: cathepsin G gained tryptic function but lost efficiency during primate evolution. J Immunol 185, 5360-8
            • ;
            • Jakubowska, AK, et al. (2010) Downregulation of a chitin deacetylase-like protein in response to baculovirus infection and its application for improving baculovirus infectivity. J Virol 84, 2547-55
            • ;
            • Zheng, H, et al. (2010) Chaperokine function of recombinant Hsp72 produced in insect cells using a baculovirus expression system is retained. J Biol Chem 285, 349-56
            • ;
            • Fielden, MR, et al. (2010) Mechanism of subendocardial cell proliferation in the rat and relevance for understanding drug-induced valvular heart disease in humans. Exp Toxicol Pathol 62, 607-13
            • ;
            • Battur, B, et al. (2009) LKR/SDH plays important roles throughout the tick life cycle including a long starvation period. PLoS One 4, e7136
            • ;
            • Li, Z, et al. (2010) Administration of a mutated myostatin propeptide to neonatal mice significantly enhances skeletal muscle growth. Mol Reprod Dev 77, 76-82
            • ;
            • Liu, H, et al. (2009) Structural characterization of the ectodomain of a disintegrin and metalloproteinase-22 (ADAM22), a neural adhesion receptor instead of metalloproteinase: insights on ADAM function. J Biol Chem 284, 29077-86
            • ;
            • Zamilpa, R, et al. (2009) C-terminal fragment of transforming growth factor beta-induced protein (TGFBIp) is required for apoptosis in human osteosarcoma cells. Matrix Biol 28, 347-53
            • ;
            • Shen, X, et al. (2009) Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics 8, 1539-51
            • ;
            • Pfalz, M, et al. (2009) The gene controlling the indole glucosinolate modifier1 quantitative trait locus alters indole glucosinolate structures and aphid resistance in Arabidopsis. Plant Cell 21, 985-99
            • ;
            • Hernandez-Rodriguez, CS, et al. (2009) Genomic structure and promoter analysis of pathogen-induced repat genes from Spodoptera exigua. Insect Mol Biol 18, 77-85
            • ;
            • Betting, DJ, et al. (2009) Enhanced immune stimulation by a therapeutic lymphoma tumor antigen vaccine produced in insect cells involves mannose receptor targeting to antigen presenting cells. Vaccine 27, 250-9
            • ;
            • Buchs, M, et al. (2009) High-throughput insect cell protein expression applicat
            • ユーザープロトコール

              タイトル
              TB359