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CBA017 InnoCyte™ Cell Migration Assay, 24-well plate

CBA017
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概要

主要スペック表

Detection Methods
Fluorometric

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CBA017-1KITCN
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      Description
      OverviewA convenient assay for chemotaxis cell migration in 24-well plates. Cells migrate through a 8 μm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye.
      Catalogue NumberCBA017
      Brand Family Calbiochem®
      Materials Required but Not Delivered 1X PBS (sterile)
      Cell culture medium (serum-free)
      Pipettors with disposable tips
      Fluorescence reader capable of measuring fluorescence in 96-well plates with a filter set of ~485 nm (excitation) and 520 nm (emission).
      Hemocytometer and microscope for cell counting
      Tissue culture facility
      Forceps
      References
      ReferencesWells, A., et al. 2002. Acta Oncol. 41, 124.
      Staff, A. C. 2001. Scand. J. Clin. Lab. Invest. 61, 257.
      Eatock, M.M., et al. 2000. Cancer Treat. Rev. 26, 19.
      Klinghoffer, R.A., et al. 1999. EMBO J. 18, 2459.
      Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677.
      Smilenov, L. B., et al. 1999. Science 286, 1172.
      Burridge, K. et al. 1997. Trends Cell Biol. 7, 342.
      Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719.
      Price, J.T., et al. 1997. Crit. Rev. Biochem. Mol. Biol. 32, 175.
      Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359.
      Product Information
      Detection methodFluorometric
      Form12 Tests
      Format24-well plate
      Kit contains12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay time3-5 h
      Sample TypeAdherent cells
      Physicochemical Information
      Emission max.
      Excitation max.
      Product Usage Statements
      Intended useThe InnoCyte™ Cell Migration Assay, 24-Well is suited for studying the effects of various drugs or agents on cell motility and for identifying chemoattractant agents. The Cell Culture Inserts have an 8-µm pore size membrane that is suitable for migration of epithelial, mesenchymal, and endothelial cell types. The membrane is not coated with an extracellular matrix protein, so is not suitable for haptotactic migration studies. The assay is not intended for leukocyte migration experiments; leucocytes require membranes with a pore size that is smaller than 8 µm.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit contains12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
      Global Trade Item Number
      カタログ番号 GTIN
      CBA017-1KITCN 07790788053628