イモビロン（Immobilon） トランスファーメンブレンシリーズ
Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.
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参考資料
| 参考資料の概要 | アプリケーション |
|---|---|
| Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923 LCGC 2010 概要を表示する 記事全文 | |
| Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green Luo S., Wehr N.B., Levine R.L. Analytical Biochemistry:350 (2006):233-238 2006 | Immunoblotting (Western) |
| Role of the Small Heat Shock Proteins in Regulating Vascular Smooth Muscle Tone McLemore E.C., Tessier D.J., Thresher J., Komalavilas P., Brophy C.M J. Am. Coll. Surg. 2005, Vol 201 (1):30-36 2005 | |
| Pre-B-cell colony-enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium. Ognjanovic S, Ku TL, Bryant-Greenwood GD. Am J Obstet Gynecol. 2005 Jul;193(1):273-82 2005 | Western Blotting |
| A high-affinity reversible protein stain for Western blots Antharavally B.S., Carter, B., Bell, P.A., Mallia K. Analytical Biochemistry 2004,Vol 329:276-280 2004 | |
| Biochemical analysis of GABA receptor subunits alpha 1, alpha 5, beta1 beta2 in the hippocampus of patients with Alzheimer's disease neurophathology Rissman, R.A., Mishizen-Eberz A.J. N.,Wolfe, C.B.B., DeBlas A.L., Miralles C.P., Ikonomovic M.D., armstrong D.M. Neuroscience 120 (2003) 295-705 2003 | Western Blotting |
| Proteomics reveals protein profile changes in doxorubicin treated MCF7 human breast cancer cells Cheng S.T., Pan T, L., Tsai Y.Ch, Huang C. M. Cancer letters 2002. vol 181:95-107 2002 | |
| Towards proteome-wide production of monoclonal antibody by phage display. Bin Liu, Lan Huang, Carina Sihlbom, Al Burlingame and James D. Marks. J Mol Biol. 2002 Feb 1;315(5):1063-73 2002 | Mass Spectrometry Sample Prep |
| Characterization of retinoic acid receptor-deficient keratinocytes. Goyette Philippe; Chen Chang Feng; Wang Wei; Seguin Francois; Lohnes David(a) Journal of Biological Chemistry v 275 pg 16497-16505 June 2, 2000 2000 | |
| Protection of renal inner medullary epithelial cells from apoptosis by hypertonic stress-induced p53 activation Dmitrieva Natalia(a); Kultz Dietmar; Michea Luis; Ferraris Joan; Burg Maurice ; Journal of Biological Chemistry v 275, pg 18243-18247 June 16, 2000 Journal of Biological Chemistry v 275, pg 18243-18247 June 16, 2000 2000 |
FAQ（よくある質問と回答）
| 質問 | 回答 |
|---|---|
| I have just received the 0.2um Immobilon-PSQ membrane. Does the membrane require any special handling procedure? | The Immobilon-PSQ can be handled in the same way as Immobilon-P. Prewetting in methanol and exchanging in MilliQ water are the only requirements prior to blotting. |
| What effect will SDS have on the New Immobilon PSQ 0.2 um membrane? | SDS interferes with the binding of protein to PVDF during transfer. Because this membrane is thicker than Immobilon P, there is a high probability that the protein will stick to the membrane. Because of the tighter pore size and increased thickness, the residence time of the protein within the membrane will favor its binding to the PVDF. If the protein is not retained well on the 0.2 um membrane, reducing the voltage or current may help as would lowering the SDS concentration ( only as a secondary step if reducing current/voltage does not work). |
| What are Millipore's suggestions to reduce blow-through with the new 0.2um Immobilon PSQ? | If blow-through is an issue, modify transfer conditions by reducing the voltage or current. |
| How much protein do I need for sequencing? | For most purposes, if the protein is visible by staining with Coomassie brilliant blue R, then there is enough protein present for sequence analysis. Check with your protein sequencing facility on the minimum requirements for their instrumentation. |
| Can the 0.2 um Immobilon PSQ membrane be used for Immunodetection ? | Immobilon- PSQ is recommended for transfering and detecting proteins of molecular weight less than 20 kD. |
| Can I use ECL with Immobilon-PSQ ? | Yes, standard chemilumniescent detection can be used with Immobilon-PSQ. Conditions used for the Immobilon-P will most likely require some optimization when switching to Immobilon-PSQ. Since the binding capacity of the Immobilon-PSQ is greater the mass of the blocking agent must be increased as well as the incubation time. You will also need to increase the wash time to ensure that the unbound anitbody is washed out of the tighter pores. Antibody concentrations may also need to be increased because the molecules are more likely to be distributed deeper into the pores. However modifying the blocking and wash steps as recommended may make adjusment of the antibody concentration unnecessary. |
| Can I use Immobilon-PSQ for all protein blotting applications? | The Immobilon-PSQ membrane should not be used as a replacement for the Immobilon P unless Immobilon-P is not working in a particular protein transfer system. Immobilon-P has demonstrated superior capabilities in applications such as standard immunodetection, rapid immunodetection, standard ECL, rapid ECL and transillumination. The use Immobilon-PSQ membrane has been shown to be most applicable in the in immunoblotting of relatively small molecular weight proteins (less than 20,000 daltons). |
| Should I prewet MultiScreen Immobilon-P plates before use? | Yes. Use 15 ul of 70% ethanol or methanol to prewet the Immobilon P membrane. This membrane is hydrophobic and requires a prewet to allow liquid to pass through the membrane. |
| What is the protein binding capacity of Immobilon-P? | The protein (BSA) binding capacity for the Immobilon P membrane is 131 micrograms per sq. cm. of membrane. |
| What is the thickness of the Immobilon PSQ? | Immobilon PSQ is 200 microns thick. |
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