IL-5 ELISPOT Antibody Pair, Human

Interleukin 5 (IL-5) is a hematopoietic growth factor that is primarily produced by activated T lymphocytes, and is approximately 70% homologically identical in both human and mouse. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, as well as stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. When production levels of this growth factor become elevated, IL-5 has also been shown to be linked to asthmatic conditions in both human and mouse models. The Millipore human IL-5 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-5 secreting cells at the single cell level.

~26 kDa

IL-5 ELISPOT Antibody Pair, Human is a highly specific rat monoclonal antibody & that targets IL-5 & has been tested in ELISA.

Research Category
Inflammation & Immunology

Both the capture and the detection antibodies included in this kit react with human interleukin -5 (IL-5).

Sufficient reagents for five, 96-well plates.

Format: Purified

The 500 µg purified IL-5 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and should be handled under aseptic conditions. The 100 µg purified IL-5 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.

Maintain refrigerated at 2-8°C for up to six months from the date of receipt.

• Human IL-5 Capture Antibody, clone TRFK5. Quantity: 500 µg.

• Human IL-5 Detection Antibody, clone JES1-5A10. Quantity: 100 µg.

• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.