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71493 HRV 3C Protease

Highly efficient fusion tag removal at low temperature

HRV 3C Protease: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
71493
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概要

Replacement Information

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カタログ番号 在庫状況包装 Qty/Pk 価格 数量
71493-10000UNCN
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      		 ｶﾞﾗｽﾋﾞﾝ 10000 un
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      71493-3CN
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      現在国内在庫なし 現在国内在庫なし
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          		 ｶﾞﾗｽﾋﾞﾝ 500 u
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          Description
          OverviewRecombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln↓GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His•Tag® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag™, thioredoxin (Trx•Tag™), glutathione-S-transferase (GST•Tag™), and NusA (Nus•Tag™) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind® metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect™ pIEx™-9 vector, and the multisystem pTriEx™-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag® II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
          Catalogue Number71493
          Brand Family Novagen®
          References
          Product Information
          Activity1.8-3.0 U/µl
          Unit of DefinitionOne unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
          •500 UHRV 3C Protease
          •10 µgHRV 3C Cleavage Control Protein
          •10 ml10X HRV 3C Cleavage Buffer
          FormulationIn 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
          Quality LevelMQ400
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          カタログ番号 GTIN
          71493-10000UNCN 04055977270259
          71493-3CN 07790788053277

          Documentation

          HRV 3C Protease 試験成績書（CoA）

          タイトルロット番号
          71493

          カタログ

          タイトル
          Bulk Product Guide
          安定したタンパク質抽出と効率的なサンプル調整に　Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品

          引用

          Title
        • Yanqiu Yuan, et al. (2007) Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Procedings of the National Academy of Science 104, 5348-5353.
          		•

          ユーザープロトコール

          タイトル
          TB420