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参考資料
| 参考資料の概要 | アプリケーション |
|---|---|
| MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues Omer Barad, Eti Meiri, Amir Avniel, Ranit Aharonov, Adi Barzilai, Isaac Bentwich, Uri Einav, Shlomit Gilad, Patrick Hurban, Yael Karov, Edward K. Lobenhofer, Eilon Sharon, Yoel M. Shiboleth, Marat Shtutman, Zvi Bentwich and Paz Einat Genome Research 14:2486-2494, 2004 2004 | |
| Highly selective membranes in protein ultrafiltration Feins M, Sirkar KK,Biotechnol Bioeng. 2004 Jun 20;86(6):603-11 Biotechnol Bioeng. 2004 Jun 20;86(6):603-11 2004 | Protein Purification |
| Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention Huisman IH (REPRINT) ; Pradanos P; Hernandez A Journal of membrane science 2000, V178, N1-2, p55-64 2000 2000 | |
| Effect of Solution pH on Protein Transport Through Ultrafiltration Membranes Douglas B. Burns and Andrew L. Zydney Biotechnol Bioeng 64: 27-37, 1999. 1999 | |
| Integrated process for the removal of emulsified oils from effluents in the steel industry Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT) Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395 1999 | Industrial QC |
| Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74 Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998 Journal of membrane science, v147, N2, p279-290 1998 1998 | Enzyme Assays |
| In vitro and in vivo grafting of xeno pig fetal liver fragments using ultrafiltration membrane Kanai N.; Morita N.; Munkhbat B.; Gansuvd B.; Hagihara M.; Nagamachi Y.;Tsuji K. Cell Transplantation SO- 7/4 (417-420) 1998 1998 | Cell Culture |
| Metabolic screening using on-line ultrafiltration mass spectrometry Van Breemen R.B.; Nikolic D.; Bolton J.L. Drug Metabolism and Disposition , 26/2 (85-90) 1998 1998 | |
| Performance Characteristics of Waste Oil Emulsion Treatment by Ultrafiltration Lin, S. H.; Lan, W. J. Yuan Ze University, Taoyuan, Taiwan J Environ Sci Health-Toxic Hazard Subst Environ Eng 1998,SO- vA33, n3, p385(20) 1998 | |
| General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification Lin, S.-C. Chen, Y.-C. Lin, Y.-M. J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998 1998 |
FAQ（よくある質問と回答）
| 質問 | 回答 | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| When doing diafiltration, how many initial volumes would it take to remove the salt? | It takes three initial volumes to remove 95% of the salt and five initial volumes to remove 99.9% of the salt. | |||||||||||||||||||||
| What molecular weight cut-off should I select for proteins of different sizes? | As a rule, when using Ultracel membranes it is best to select a membrane 2-3 times smaller than the solute to be retained by the membrane. In the case of the Biomax membranes 4-5 times smaller than the NMWL is suggested. For more information on choosing the appropriate membrane cutt off: http://www.millipore.com/publications.nsf/docs/RF036 | |||||||||||||||||||||
| What is the best way to store disc membranes? | Solutions of 10% ethanol in water or 0.1% azide in water will preserve the membrane. For long-term storage, refrigeration is recommended. | |||||||||||||||||||||
| What is the pH range for YM membrane? | The pH range is 3-13. | |||||||||||||||||||||
| My recovery is low. Why? | 1. What concentration are you using? a. If it is protein and <10ug/ml, the protein may be binding to the plastic (not the membrane). We suggest passivation (See passivation protocol). b. If it is DNA and >100ug/ml, most likely the DNA is precipitating out. There is no solution except use less DNA. 2. Are you using a detergent? a. If yes, and it is greater than the critical micelle concentration, it will not pass through the membrane pores. Suggest diluting out the sample (See detergent study). |
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| What is the temperature limit for the Ultracel YM membrane? | The temperature limit for the Ultracel YM membrane is 121 degrees C. | |||||||||||||||||||||
| What is the temperature limit for the Biomax membrane? | The temperature limit for the Biomax membrane is 50 degrees C. | |||||||||||||||||||||
| What is the average thickness of the Ultracel YM membrane? | The average thichness of the Ultracel YM membrane is 8 mil. | |||||||||||||||||||||
| What is the difference between Biomax and Ultracel membrane? | Biomax is a polyethersulfone membrane designed with maximum flow characteristics. The protein retention is typically 80-85% of a globular solute with that molecular weight limit. Ultracel is a regenerated cellulose membrane designed with maximum recovery characteristics. The protein retention is typically 90-95% of a globular solute with that molecular weight limit. | |||||||||||||||||||||
| What are the different membranes types and advantages? | MEMBRANE TYPE & MW Cut-off
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関連製品: Application Facete
| Protein Purification |
| Dialysis and Filtration |
関連製品: Brand Facete
| Ultracel® |
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| Reagents, Chemicals and Labware > Lab Filtration > Ultrafiltration Membrane Discs |