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69067 Enterokinase Cleavage Capture Kit

Enterokinase Cleavage Capture Kit: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
69067
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概要

Replacement Information

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69067-3CN
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      Description
      OverviewThe Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase.
      Following cleavage of the target protein, rEK is removed with > 99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration. Because the same buffer conditions are used for both cleavage and capture, no buffer changes are necessary.

      The kit also includes a Cleavage Control Protein for conducting control digests in parallel with experimental samples, or to test cleavage under customized buffer conditions. The 48 kDa Cleavage Control Protein is cleaved into two proteolytic fragments of 32 kDa and 16 kDa, which are easily visualized by standard SDS-PAGE followed by Coomassie blue staining (see figure below). The Cleavage Control Protein also features an amino terminal S•Tag™ sequence enabling sensitive detection of the 16 kDa proteolytic product with Western Blot reagents. The Cleavage Control Protein is also available separately.
      Catalogue Number69067
      Brand Family Novagen®
      References
      References1. Collins-Racie, L.A., McColgan, J.M., Grant, K.L., DiBlasio-Smith, E.A., McCoy, J.M., and LaVallie, E.R. (1995) Bio/Technology 13, 982–987.
      Product Information
      Components
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      69067-3CN 07790788056346

      Documentation

      Enterokinase Cleavage Capture Kit 試験成績書（CoA）

      タイトルロット番号
      69067

      参考資料

      参考資料の概要
      1. Collins-Racie, L.A., McColgan, J.M., Grant, K.L., DiBlasio-Smith, E.A., McCoy, J.M., and LaVallie, E.R. (1995) Bio/Technology 13, 982–987.

      引用

      Title
    • Wu. L., et al. (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M J. Immunol. 183, 421.
    • Marcelo Comini, et al. (2005) Trypanothione synthesis in Crithidia revisited. Journal of Biological Chemistry 280, 6850-6860.
    • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
    • Changlu Liu, et al. (2005) INSL5 is a high affinity specific agonist for GPCR142 (GPR100). Journal of Biological Chemistry 280, 292-300.
    • Deanne M. Compaan and W. Ross Ellington. (2003) Functional consequences of a gene duplication and fusion event in an arginine kinase. 206, 1545-1556.
    • Gerald M. Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A+U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure. Journal of Biological Chemistry 278, 33039-33048.
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      タイトル
      TB150