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324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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324715
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概要

Replacement Information

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324715-5UGCN
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      Description
      OverviewNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
      Catalogue Number324715
      Brand Family Calbiochem®
      References
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Product Information
      CAS number72561-05-8
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
      EC number3.4.21.50
      FormLyophilized
      FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥200 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      324715-5UGCN 04055977215960

      Documentation

      Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes 試験成績書（CoA）

      タイトルロット番号
      324715

      参考資料

      参考資料の概要
      Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-February-2012 RFH
      DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
      FormLyophilized
      FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
      Recommended reaction conditions
      Protocol 1. Dissolve the proteins to be sequenced in 25 mM Tris-HCl, pH 8.5, and 1 mM EDTA. For proteins that are difficult to solubilize, add urea, SDS, or guanidine-HCl to the digestion buffer prior to solubilization. If urea is used, add 20 mmol/l of methylamine. 2. The suggested working concentration is 1:20-1:100 (protease:protein by weight). 3. Incubate for 2-18 h at 37°C.
      CAS number72561-05-8
      EC number3.4.21.50
      Purity≥90% by SDS-PAGE
      Specific activity≥200 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
      SolubilityReconstitute in 50 µl of distilled H₂O.
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Toxicity Harmful
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

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      カテゴリー

      Life Science Research > タンパク質と酵素 > Other Enzymes